学术
CD1D基因与B7—1基因共转染胰腺癌细胞及其抗癌作用的实验研究
王昆华 龚昆梅 张勇学 钟鸣 欧阳一鸣 凌平 黄映光 张剑 朱宇 刘为军 赵喜荣
云南省第一人民医院普通外科,云南昆明650032
摘 要:
目的观察共转染小鼠B7-1和CD1D基因对小鼠胰腺癌细胞的免疫应答。方法将表达B7-1和CD1D基因的逆转录病毒载体导入包装细胞系，然后转染入胰腺癌细胞。用PCR和Westem方法鉴定细胞表达；用B7-1和CD1D阳性的细胞在体内诱导抗肿瘤免疫反应。结果B7-1和CD1D阳性的细胞在同基因小鼠体内能诱导抗肿瘤免疫反应并抑制肿瘤生长。结论B7-1基因和CD1D基因共转染肿瘤细胞，能抑制肿瘤生长，可能为肿瘤的基因治疗开辟一条新途径。[著者文摘]
文章出处:
《中国普通外科杂志》-2007年16卷5期 -442-445页
栏目信息:
文献标识码:
A
文章编号:
1005-6947（2007）05-0442-04
Study of cotransfection of B7-1 gene and CD1D gene in pancreatic carcinoma cell and its anti-tumor responses in mice
WANG Kun-hua, GONG Kun-mei, ZHANG Yong-xue, ZHONG Ming, OUYANG Yi-ming, LIN Ping, HUANG Ying-guang, ZHANG Jian, ZHU Yu, LIU Wei-jun, ZHAO Xi-rong （ Department of General Surgery, the First People＇s Hospital of Yunnan Province, Kunming 650032, China ）
Abstract:
Objective To study the cotransfection roB7-1 and mCD 1D gene into pancreatic cancer cells of rats and to observe its anti-tmor responses. Methods Recombinant retroviral vectors expressing mB7-1 and mCD1D gene were packaged into GP2 - 293 cell lines and transfected. The expressions of mB7-1 and mCD1D were detected with PCR and Western blot. The positive cells of mB7-1 and mCD1D were used to induce the anti-tumor immunity in vitro. Results Anti-tumor immunity was induced after B7-1 and CD1D positive cells were coinoculated in syngeneic mice. Furthermore, the growth of tumor was inhibited. Conclusions Cotransfection of B7-1 and CD1D could induce anti-tumor effect. This study provide a foundation for the application of B7-1 and CD1D gene therapy in tumor.[著者文摘]
Key words:
Genes, CD1D ; Genes, B7-1 ; Transfection ; Pancreatic Carcinoma Cell
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