学术
EGFR基因C端结构域真核表达载体的构建
李静[1] 文中伟[2] 李燕[1] 周建华[3] 陈晓光[1]
[1]中国协和医科大学中国医学科学院药物研究所,北京100050 [2]中国协和医科大学中国医学科学院基础研究所,北京100050 [3]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076
摘 要:
构建EGFR基因C端结构域的真核表达载体.应用PCR技术,从含EGFR基因C端结构域的大肠杆菌DH 5α中扩增其序列,亚克隆到真核表达载体pcDNA3.1（＋）中,经酶切及测序进行验证.PCR扩增片段与预期结果相符,真核表达载体构建成功,测序结果与GenBank公布的基因一致.成功地构建了EGFR基因C端两个结构域的真核表达载体.[著者文摘]
文章出处:
《哈尔滨商业大学学报：自然科学版》-2007年23卷3期,264 -257-260,264页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1672-0946（2007）03-0257-04
Construction of eukaryotic expression vector for EGFR gene C-terminal domain
LI Jing , WEN Zhong-wei, LI Yan, ZHOU Jian-hua ,CHEN Xiao-guang （1. Institute of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 10050, China;2. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100050, China;3. Research Center on Life and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China）
Abstract:
To construct the eukaryotic expression vector containing EGFR gene C -terminal domain. EGFR gene C - terminal domain from DH 5α is cloned by PCR, and subclons it into pcDNA 3.1 （ ＋ ） vector. The recombinant vector is vested by double-enzyme digestion and sequencing. The target fragment （1158 bp） is obtained as expected, pcDNA3.1 （ ＋ ） hEGFR eukaryotic expression vector was successfully constructed, sequence analysis of the inserted target fragment revealed the same sequence as that published in Gen Bank. The pcDNA3.1 （ ＋ ）-hEGFR eukaryotic expression vector was successfully constructed.[著者文摘]
Key words:
epidermal growth factor receptor; PCR technology; eukaryotic expression vector; construction
更多>>免费文章
cqvip.com