学术
中国主栽香蕉品种和INIBAP引进品种的SSR分析研究
宁淑萍[1,4] 许林兵[2] 魏平[1] 葛学军[3]
[1]中国科学院华南植物园,广州510650 [2]广东省农业科学院果树研究所,广州510640 [3]中山大学生命科学学院,广州510275 [4]中国科学院研究生院,北京100049
摘 要:
得用10对SSR引物对中国14个主栽香蕉（Musa spp．）品种和从INIBAP引进的33个香蕉品种进行了遗传多样性分析。10个多态性位点共揭示出92个等位基因，每个位点的等位基因数从5到15不等，平均每个位点的等位基因数是9．2，产物片段大小在75bp到310bp之间。用Jaccard系数计算品种间的相似性，相似性数值在0．1到1之间；用UPGMA进行聚类分析，结果显示，14个主栽品种的遗传变异小，而供试的33个引进品种遗传多样性高。本研究所用的lO对引物不能把所有的品种区分开。[著者文摘]
文章出处:
《热带亚热带植物学报》-2007年15卷1期 -16-22页
分 类 号:
文献标识码:
A
文章编号:
1005-3395（2007）01-0016-07
Genetic Diversity of Chinese Main Banana Cultivars （Musa spp.） and Introduced Accessions from INIBAP Using Simple Sequence Repeats （SSRs）
NING Shu-ping, XU Lin-bing, WEI Ping, GE Xue-jun. （1. South China Botanical Garden, the Chinese Academy of Sciences, Ouangzhou 510650, China; 2. Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. School of Life Science, Sun Yat-Sen University, Guangzhou 510275, China; 4. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China）
Abstract:
Microsatellites were used to investigate the genetic diversity and relationships among 14 Chinese main banana cultivars （Musa spp.） and 33 introduced accessions from International Network for the Improvement of Banana and Plantain （INIBAP）. Ten out of forty-seven microsatellite primer pairs amplified reproducible and discrete DNA bands, revealing a total of 92 alleles. The average number of alleles amplified by per primer was 9.2, ranging from 5 to 15. The sizes of PCR products were between 75 bp and 310 bp. The genetic similarity among the cultivars was estimated by the Jaccard coefficient, and the similarity value was between 0.1 and 1. Relationships among the cultivars were evaluated using the unweighted pair-grouping with arithmetic average （UPGMA） clustering. The dendrogram showed that the introduced accessions presented higher levels of genetic diversity than the main cultivars. All the cultivars tested could not be discriminated by the 10 primers.[著者文摘]
Key words:
Banana; Genetic diversity; SSR
基金资助:
致谢 广东省农业科学院果树研究所国家果树种质广州香蕉圃提供试验材料,特此致谢!
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