学术
杜鹃红山茶遗传多样性的ISSR分析
罗晓莹[1] 庄雪影[1] 杨跃生[2]
[1]华南农业大学林学院,广州510642 [2]华南农业大学生命科学学院,广州510642
摘 要:
运用ISSR分子标记技术，利用筛选的10条ISSR引物，对珍稀濒危植物杜鹃红山茶（Camellia changii Ye）2个亚种群60个单株的遗传多样性进行了研究。结果表明：物种水平上的多态位点百分率（PPB）为55．29％，Nei’s基因多样性指数（H）及Shannon多态性信息指数（T）分别为0．2191和0．3215。种群间的遗传分化系数（GST）仅为0．0922。研究结果揭示了杜鹃红山茶的遗传多样性较低，亚种群间遗传分化较小。小种群和人为活动干扰是杜鹃红山茶现存种群的主要限制因素。影响杜鹃红山茶种群发展的其它因素亟需进一步研究。[著者文摘]
文章出处:
《热带亚热带植物学报》-2007年15卷2期 -93-100页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1005-3395（2007）02-0093-08
Genetic Diversity of Camellia changii Ye （Theaceae） Using ISSR Markers
LUO Xiao-ying, ZHUANG Xue-ying, YANG Yue-sheng （1. Forestry College, South China Agricultural University, Guangzhou 510642, China; 2. Life Science College, South China Agricultural University, Guangzhou 510642, China）
Abstract:
Camellia changii Ye is a rare and endangered species endemic to China. It is confined to the E＇huangzhang Nature Reserve at Yangchun City, southwestern Guangdong Province, South China. The genetic diversity was studied using Inter-Simple Sequence Repeat （ISSR） markers. A total of 60 individuals from two subpopulations were sampled. Ten primers with discernible DNA bands were applied. The percentage of polymorphic bands （PPB）, Nei＇s Index and Shannon＇s Index were 55.29%, 0.2191 and 0.3215, respectively. The coefficient ofgene differentiation （Gsr） was 0.0922. These results indicate that C. changii has low genetic diversity and low differentiation between the two subpopulations. Small population size and human disturbances are the main limiting factors for the extant population. Further studies on the factors limiting population development are urgently required.[著者文摘]
Key words:
Camellia changii; Endemic species; Genetic diversity; ISSR
基金资助:
Foundation item： Supported by the Forestry Bureau Science and Technology Project of Guangdong Province.Acknowledgements： We thank the E＇huangzhang Provincial Nature Reserve of Guangdong Province and Yangchun Forestry Bureau for their assistance in field work. We also thank Dr. Richard T. Corlett for his helpful comments on early version of the manuscript.
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