学术
在Fas诱导Bel-7402细胞凋亡中p38MAPK调节Bcl-2的表达
王玉[1,2] 孙黎光[1] 夏春辉[2] 叶丽平[1] 张莹[1]
[1]中国医科大学基础医学院生物化学与分子生物学教研室,辽宁省沈阳市110001 [2]齐齐哈尔医学院,黑龙江省齐齐哈尔市161042
摘 要:
目的：探讨p38MPAK是否参与Fas和AD诱导Bel-7402细胞的凋亡过程。以及p38MPAK和bcl-2的关系，进一步揭示p38MAPK的凋亡途径．方法：在Fas和AD作用24h后，用MTT法检测Bel-7402细胞的活力。用Western—blot和RTPCR法检测p38MAPK，p-p38MAPK和Bcl-2expression，用免疫荧光法对p—p38MAPK进行细胞定位．结果：随着Fas浓度的增加，Bel-7402细胞的活力明显抑制，p38MAPK和p-p38MAPK表达明显增高（P〈0．01），且p-p38MAPK由胞质易位到胞核、Bcl-2的表达明显降低（P〈0．01），并且这种降低趋势被p38MAPK抑制剂SB203580所阻止结论：p38MAPK参与Fas诱导的凋亡途径，以磷酸化形式激活后抑制Bcl-2的表达，进而促进细胞凋亡．[著者文摘]
文章出处:
《世界华人消化杂志》-2007年15卷30期 -3184-3189页
栏目信息:
分 类 号:
p38 mitogen-activated protein kinase modulates Bcl-2 expression during FAS-induced apoptosis in Bel-7402 cells
Yu Wang, Li-Guang Sun, Chun-Hui Xia, Li-Ping Ye, Ying Zhang（Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, Liaoning Province, China; Qiqihar Medical College, Qiqi- har 161042, Heilongjiang Province, China）
Abstract:
AIM： To investigate whether p38 mitogenactivated protein kinase （p38MAPK） is involved in Fasand actinomycin D （AD）-induced apoptosis in Bel-7402 cells, and the relationship between p38MAPK and Bcl-2 expression. METHODS： We measured the viability of Bel-7402 cells by MTT assay, p38MAPK, p-p38MAPK and Bcl-2 expression by Western blotting and reverse transcription polymerase chain reaction （RT-PCR）, and the location ofp-p38MAPK in Bel-7402 cells after Fas and AD treatment by immunofluorescene. RESULTS： Bel-7402 cell viability was significantly inhibited by Fas （P 〈 0.01）. p38MAPK and p-p38MAPK increased significantly with increasing Fas （P 〈 0.01）, leading to cell death as assessed by MTT after 24 hours of Fas and AD treatment, p-p38MAPK translocation into the nucleus was dependent on Fas stimulation. Bcl-2 expression decreased significantly and was prevented by SB203580 during Fas- and ADinduced apoptosis （P 〈 0.01）. CONCLUSION： p38MAPK is involved in Fasand AD-induced apoptosis, and p38MAPK modulates Bcl-2 expression during this apoptosis pathway.[著者文摘]
Key words:
p38 mitogen-activated protein kinase;p-p38 mitogen-activated protein kinase; Bcl-2;Apoptosis; Western blotting; Reverse transcriptionpolymerase chain reaction
基金资助:
辽宁省教育厅科研基金资助项目,No.2004D173黑龙江省普通高等学校骨干教师创新能力资助计划,No.1054G070
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