学术
摘 要:
利用分子生物学技术，直接从化学-生物絮凝工艺的活性污泥样品中提取DNA，对16S rDNA V3区进行PCR扩增，结合DGGE（变性浓度梯度凝胶电泳），分析了活性污泥中微生物群落结构，并对Shannon多样性指数进行分析讨论，通过研究指出系统中细菌数量的增加或减少．测定了活性污泥中部分菌种的16S rDNA V3区片段序列，通过NCBI（美国国立生物技术信息中心）基因库比对，初步确定细菌的属．结果表明，PCR—DGGE结合测序技术是一种完全可行的快速进行环境样品微生物研究的分析方法．图3表4参13[著者文摘]
关 键 词:
文章出处:
《应用与环境生物学报》-2006年12卷1期 -108-112页
栏目信息:
Analysis of Community Structure in Chemical and Biological Flocculation Wastewater Treatment Process
SHI Yan, FU Yigang, XIA Siqing, ZHAO Jianfu （State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongfi University, Shanghai 200092, China）
Abstract:
The microbial community structure in chemical and biological flocculation wastewater treatment process was analyzed with molecular biological methods, such as DNA extraction, polymerase chain reaction （PCR） and denaturting gradient gel electrophoresis （DGGE）, and the Shannon diversity index were also calculated and discussed. The increase or decrease in bacteria was indicated by this analysis. The sequences of several 16S rDNA DGGE fragments were determined and some possible genera of bacteria were confirmed by comparison with GeneBank（NCB1）. The result shows that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis of environmental samples. Fig 3, Tab 4, Ref 13[著者文摘]
Key words:
PCR; DGGE; activated sludgc; microbial community structure; Shannon diversity index
基金资助:
国家“863计划”资助项目（2002AA601320）和上海市曙光计划资助项目
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