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Single-step Purification of Molecular Chaperone GroEL by Expanded Bed Chromatography

《中国化学工程学报:英文版》2003年 第4期 | 佟晓冬 杨征 董晓燕 孙彦   DepartmentofBiochemicalEngineering SchoolofChemicalEngineeringandTechnology TianjinUniversity Tian-jin300072 China
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摘 要:Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important protein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarified homogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provided a single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and column washing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bed mode in the purification of GroEL from cell disruptate.
【分 类】【工业技术】 > 化学工业 > 一般性问题 > 化工过程(物理过程及物理化学过程) > 分离过程 > 单相系气体混合物的分离过程 > 吸附和脱吸附
【关键词】 膨胀床 吸附技术 纯化 分子伴侣 GROEL 分离
【出 处】 《中国化学工程学报:英文版》2003年 第4期 460-463页 共4页
【收 录】 中文科技期刊数据库