学术
摘 要:
氨基酸是构成蛋白质的基本单位，是生物体内不可缺少的营养成分，L-亮氨酸是人体8种必需氨基酸之一，也是三种支链氨基酸之一，在医药、食品、饲料、化妆品等行业具有重要的用途。以一株黄色短杆菌为出发菌株，经紫外线、盐酸羟胺和硫酸二乙酯等多次诱变处理，单菌落分离、摇瓶初筛和摇瓶复筛，获得了一株蛋氨酸和异亮氨酸双重缺陷型突变株SFL8-3（Met^-，Ile^-），摇瓶发酵L-亮氨酸产量达18．98g／L。[著者文摘]
文章出处:
《中国食品添加剂》-2006年4期 -123-128页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1006-2513（2006）04-0123-06
Screening of producing L- leucine brevibacterium flavum mutant With L- Isoleucine and methionine auxotroph
LIU Jian-jun ZHAO Xiang-ying LI Pi-wu TIAN Yan-jun ZHANG Jia-xiang （ Food ＆ Fermentation Engineering Key Lab of Shandong Province, Jinan 250013）
Abstract:
Amine acids are the fundamental unit of protein molecules and the necessary nutrition composition in organisms. L - leucine is one of 8 kinds of essential amine acids, and one of 3 kinds of branched chain ones. L - leucine acid has been widely applied in medicine, nurture food, etc. After Brevibacterium flavum SFL - 8 was mutagenized repetitiously with UV, HC1. NH4OH, diethylsulphate and LiC1 single or multiple treat, the mutant strain Brevibacte- rium flavum SFL8 -3 with L - Isoleucine and methionine auxotroph was obtained by shaking flask fermentation selection. This strain could produce 19. 98g/L L - leucine from 10% glucose by shaking flask fermentation.[著者文摘]
Key words:
brevibacterium flavum; L - leucine; auxotroph ; fermentation
基金资助:
山东省科技攻关计划（N0.012100106）
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