学术
国际标准刊号：ISSN 1006-2513
国内统一刊号：CN 11-3542
摘 要:
建立虾青素的含量及有关物质的反相高效液相色谱测定方法。采用Discovery-C18（4.6mm×250mm,5μm）色谱柱,以甲醇-丙酮-水（76∶15∶9）为流动相,流速为1.0mL/min,检测波长为482nm,柱温为30℃。在所选定的液相色谱条件下,有关物质与主药分离良好,虾青素在636μg/mL范围内线性良好,检出灵敏度为11ng/mL,平均回收率为100%,RSD为0.29%。[著者文摘]
文章出处:
《中国食品添加剂》-2007年4期 -151-152页
China Food Additives
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1006-2513（2007）04-0151-02
[参考文献]
Determination of astaxanthin and its related substances by HPLC
ZHANG Yu.zhen,LIU Zhe （ Zhejiang Medicine Co. , LTD. Xinchang Pharmaceutical Factory, Zhejiang, Xinchang 312500）
Abstract:
A HPLC method for determination of Astaxanthin and its related substances was established. A C,scolumn was used with the mobile phase of methanol-acetone-water（76 ： 15 ： 9）at the detection wavelength of 482 nm. The calibration curve was linear in the range of 6-36μg/mL. The detection limit of Astaxanthin were 11 ng. The average recovery was 100% with RSD of 0. 29%.[著者文摘]
Key words:
astaxanthin ; related substance; HPLC ; determination
收稿日期: 2007-03-09
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