学术
摘 要:
以芦丁为标样，选择测定波长为502nm，用分光光度法测定鱼腥草叶总黄酮含量，结果表明：芦丁浓度在8.1248.72μg/mL范围内，吸光度和浓度呈良好的线性关系（R^2=0.9998），测定的平均回收率为99.4%，精密度试验RSD=0.49%，重现性试验RSD=2.07%。该方法精密度高，结果稳定可靠。此外用纸色谱和显色反应对鱼腥草叶黄酮作了初步鉴别。[著者文摘]
文章出处:
《中国食品添加剂》-2007年5期 -143-147页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1006-2513（2007）05-0143-05
Determination and identification of flavonoids in leaves of houttuynia cordata Thunb
YE Chun, XU Li-juan, YANG Gen-qin, YANG Cong-xian （College of Life Science, Guizhou University, Guiyang 550025, China）
Abstract:
Total flavonoids content in the leaves of Houttuynia cordata Thunb was determined by Spectrophotometry at the wavelength of 502nm, with rutin as standard sample. The determination result showed： there was a linear relationship between the concentration of the rutin solution and absorption spectrum in the range of 8. 12 -48. 72μg g/mL （R^2 =0. 9998）. The average recovery rate of this method was 99. 4%, the RSD of precision test was 0. 49% and the RSD of reproducibility test was 2.07%. This method was good in precision and reproducibility. Furthermore, the preliminary identification of the leaves of Houttuynia cordata Thunb was done by paper chromatography and color reaction.[著者文摘]
Key words:
houttuynia cordata Thunb. leaves; flavonoids ; spectrophotometry; identification
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