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IDENTIFICATION OF GLUCOSE TRANSPORTER－1 AND ITS FUNCTIONAL ASSAY IN MOUSE GLOMERULAR MESANGIAL CELLS CULTURED IN VITRO
章精 刘志红 刘栋 黎磊石 ResearchInstituteofNephrology JinglingHospital NanjingUniversitySchoolofMedicine Nanjing210002
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摘要：Objective. To evaluate the role of glucose transporter-1 (GLUT1) in the glucose uptake of glomerular mesanglal cells. Methods. Cultured C57/SJL mouse mesangial cells were used in the study. The expression of GLUT1 mRNA was detected by RT-PCR. The expression of GLUT1 protein was detected by immunofluorescence and flow cytometry. The uptake of glucose and its kinetics were determined by 2-deoxy-[3H] -D-glucose uptake. Results. Both GLUT1 mRNA and protein were found in mouse glomerular mesangial cells. 2-deoxy-D-glucose uptake and kinetics assay showed that this glucose transporter had high affinity for glucose and the glucose uptake specificity was further confirmed by phloretin. Conclusion. Functional GLUT1 did present in mouse mesangial cells cultured in vitro and it might be the predominant transporter mediated the uptake of glucose into mesangial cells. | |
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| 格式：PDF 页数：5 页 页码范围：35-39页 | ||
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| 关 键 词：糖尿病肾病 肾小球系膜细胞 葡萄糖载体 荧光免疫试验 血细胞计数 | ||
| 来源期刊：《中国医学科学杂志：英文版》2001年 第1期 | ||
| 收录数据库：中文科技期刊数据库 | ||
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