学术
基于线粒体cyt b基因标记探讨凤鲚3群体遗传结构和进化特征
程起群[1,2] 马春艳[1,3] 庄平[1] 沙珍霞[4] 陆星[1] 缪炯[1]
[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点实验室,上海200090 [2]复旦大学生物医学研究院,上海200032 [3]华东师范大学生命科学学院,上海200062 [4]中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点实验室,山东青岛266071
摘 要:
为阐明凤鲚不同地理群体的遗传结构和进化关系，采用凤鲚长江（21尾）、闽江（22尾）和珠江（22尾）群体的线粒体DNA（mitochondrial DNA，mtDNA）的细胞色素b（cytb）基因片段序列进行分析。共获得3群体cyt b基因片断607 bp的一致序列。序列有102个变异位点，总变异率为16．8％，其中68个为简约位点。各单倍型的变异全部是转换或颠换，无插入和缺失。（A＋T）含量为57．6％，大于（G＋C）的含量42．4％。核苷酸多态性以闽江群体最高，其它两群体较低。自然选择检验显示3群体内部在分子水平上存在自然选择作用。在所获得的65个序列中，共检测到34种单倍型。群体内的遗传距离为0．3％～1．2％之间，群体间遗传距离在0．8％～10．8％之间。长江群体与珠江群体之间的遗传距离较大为10．8％，长江群体与闽江群体间的遗传距离为10．6％，而珠江和闽江群体之间的遗传距离最小为0．8％。AMOVA分析显示，群体间遗传变异占总变异的90．25％，群体内的遗传变异占总变异的9．75％，提示群体间变异是总变异的主要来源。研究结果提示长江群体和闽江以及珠江群体间的分化可能已达亚种水平。[著者文摘]
文章出处:
《水产学报》-2008年1期 -1-7页
分 类 号:
文献标识码:
A
文章编号:
1000-0615（2008）01-0001-07
Genetic structure and evolution characters in three populations of Coilia mystus based on cytochrome b gene segment sequence of mitochondrial DNA
CHENG Qi-qun, MA Chun-yan, ZHUANG Ping, SHA Zhen-xia,LU Xing, MIAO Jiong （1.Key Laboratory of Marine and Estuarine Fisheries Certificated by the Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; 3.School of Life Science, East China Normal University, Shanghai 200062, China ; 4. Key Laboratory of Sustainable Utilization of Marine Fishery Resources Certificated by the Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China）
Abstract:
To clarify the genetic structtLre and evolutionary characters in three populations of Coilia mystus, the mitochondrial DNA cyt b gene was used in this study. A total of 65 fish, i.e., 21 individuals from the Yangtze River estuary, 22 from the Minjiang River estuary, and 22 from the Pearl River estuary, were collected. Sequences of cyt b gene segments amplified by PCR were used. 607 consensus sequences of cyt b were obtained. Thereinto, 102 were variable sites, accounting for 16.8% of total sequence, and 68 were parsimony sites. They were all transitional and transversional variations, and no indels were found. The content of A ＋ T（57.6%） is bigger than G ＋ C（42.4% ）. The index of nucleotide diversity is largest in the Minjiang population among these three populations. Selection tests disclose that natural selection operates at molecular level within these three populations. In the total 65 sequences, 34 distinct haplotypes were detected. Genetic distance within the populations were between 0.3% and 1.2%, and between populations were from 0.8% to 10.8%. The largest genetic distance was 10.8% between the Yangzte River population and the Pearl River population, and the smallest was 0.8% between the Minjiang River population and the Pearl River population. AMOVA analysis disclosed that variation between populations accounting for 90.25% of total variation, suggesting between population variations are the main source of total variation. This study suggests that the genetic divergence between the Yangtze River population and the Minjiang River population, the Pearl River population may be arrived at subsoecies level.[著者文摘]
Key words:
Coilia mystus; cytochrome b gene; genetic diversity; genetic differentiation
基金资助:
国家自然科学基金（30600456）;中国博士后科学基金项目（20060400622）;农业部海洋渔业资源可持续利用重点实验室开放课题（实开-2005-06）
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