摘 要:
对我国长江与移居到英国泰晤士河、美国旧金山湾的3个中华绒螯蟹群体的线粒体细胞色素氧化酶亚基Ⅱ（COⅡ）的全序列进行了测定。在693bp的序列长度中，长江群体、泰晤士河群体和旧金山湾群体分别有20、8、7个变异位点；它们的单倍型多样性指数分别为0．8433、0．8158、0．7821；核苷酸多样性指数分别为0．0048、0．0031、0．0039。AMOVA分析表明：长江群体与泰晤士河群体和旧金山湾群体已出了显著的遗传分化，而泰晤士河群体和旧金山湾群体间却未出显著的遗传分化。邻接法（NJ）构建的系统关系树和最小拓展网络分析显示，旧金山湾群体与泰晤士河群体存在一定程度的基因流，旧金山湾的中华绒螯蟹群体除直接来自中国长江外，也可能有一部分来自先移居于欧洲的群体。[著者文摘]
文章出处:
《水产学报》-2008年1期 -8-12页
分 类 号:
文献标识码:
A
文章编号:
1000-0615（2008）01-0008-05
Analysis of mtDNA CO Ⅱ sequence and phylogeny in three populations of Chinese mitten crab, Eriocheir sinensis
WANG Cheng-hui, LI Si-fa, LIU Zhi-zhi, GONG Xiao-ling, HUANG Lei, SONG Xiao, ZHAO Yan （Key laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China）
Abstract:
In this paper, mitochondrial CO Ⅱ gene sequences with 693 bp were analyzed from Chinese mitten crab （Eriocheir sinensis） of the Yangtze Rive in China, the Thames River in the United Kingdom and the San Francisco Bay in U. S.A. 20 variable sites were found in the Yangtze River population, 8 and 7 variable sites were found in the Thames River and the San Francisco Bay populations, respectively. The haplotype diversities for the Yangtze River, Thames River and San Francisco Bay populations were 0. 8433, 0. 8158 and 0. 7821 respectively, and the nucleotide diversities were 0.0048, 0. 0031 and 0.0039, respectively. AMOVA analysis showed that significant genetic divergence existed in populations of the Yangtze River with Thames River and San Francisco Bay, but there was not significant divergence between the Thames River and San Francisco Bay populations. Phylogenetic tree based on NJ methods and the analysis of Minimum Spanning Network all indicated that there would have occurred gene flow between the Times fiver and the San Francisco Bay populations. The results showed that the San Francisco Bay population could have originated from both China and Europe.[著者文摘]
Key words:
Eriocheir sinensis; Yangtze River; Thames River; San Francisco Bay; COⅡ gene
基金资助:
上海市科委重大科技攻关项目（04DZ19306）;上海市农委科技兴农重点攻关项目（沪农科攻字2004第8-7号）;上海市自然科学基金项目（04ZR14123）;上海市重点学科建设项目（Y1101）
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