学术
三倍体和二倍体银鲫精巢组织学的比较
杨洁[1] 陈伟兴[1] 吴宏达[2] 范兆廷[1]
[1]东北农业大学水产养殖系,黑龙江哈尔滨150030 [2]黑龙江省农业科学院畜牧研究中心,黑龙江哈尔滨150086
摘 要:
通过光学显微镜对染色体数为150±的三倍体和染色体数为100的二倍体银鲫（Carassius auratus gibelio Bloch）的精巢组织学结构进行了比较观察。三倍体和二倍体银鲫的精巢组织学结构基本相同，属于小叶型，都是由外膜和实质构成，各精小叶呈辐射状分布，一个小叶由数个精小囊组成。其精原细胞存在于精小叶内壁上，精母细胞和未成熟的精子细胞位于精小囊中，精子成熟后从精小囊进入小叶腔。三倍体银鲫成熟精子的体积为（11．8±2．8）μm^3，二倍体平均为（6．8±1．8）μm^3，二者的比例接近3：2。结果表明三倍体银鲫的精巢能够发育成熟，其精子发生过程正常，经过减数分裂，能产生正常的精子；因此三倍体的黑龙江银鲫是具有双倍性特征的多倍体群体。[著者文摘]
文章出处:
《水产学报》-2008年1期 -21-26页
分 类 号:
文献标识码:
A
文章编号:
1000-0615（2008）00-0021-06
Comparative study on the spermary histological structure of triploid and diploid Carassius auratus gibelio
YANG Jie,CHEN Wei-xing,WU Hong-da,FAN Zhao-ting（1.Department of Aquaculture, Northeast Agriculture University, Harbin 150030, China; 2. Animal Husbandry Research Center, Heilonjiang Academy of Agricultural Sciences, Harbin 150086, China）
Abstract:
The curcian carp （Carassius auratus gibelio Bloch） is distributed mainly over Europe, northeast and northwest China. In northeast China, especially in Heilongjiang water, the crucian carp has two subspecies. The one was considered to be troploid that has 150 ± chromosomes in its somatic cells, and reproduces by gynogenesis. There are only some 5%-25% males in this population. The other was regarded as diploid whose somatic cells has 100± chromosomes. Spermary histological structures of triploid and diploid in crucian carp （ Carassius auratus gibelio Bloch） were investigated by means of light microscope. The results indicated no abnormalities were found in spermatogenesis of the triploid, and its spermary which is similar to the diploid is lobular type which constituted by out membrane and parenchyma. The seminiferous lobulas are composed of several cysts and distribute in radiation. The spermatocyte and immature spermatid located in the spermatogenic cysts, after sperma-tozoa have matured, the spermatozoa were released into lumen of seminiferous lobula out of the spermatogenic cysts. There were several leydig cells among seminiferous lobulas. However, mature spermatozoa in lobule lumen of the triploid were obviously less than those in the diploid. The crucian carp may have reproduced gynogeneticaly before diploidization but has now become diploid in behaviour. The change of reproductive form might be a long transition period. The triploid is fertile and can form mature spermatozoa after meiosis, which was regarded as triploid would be hexaploid or is undergoing the course of diploidization.[著者文摘]
Key words:
Carassius auratus gibelio Bloch; spermary; triploid; diploid
基金资助:
农业部北方鱼类生物工程育种重点开放实验室开放课题（B019200306）
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