学术
北极海冰低温微生物的生长条件及其胞外水解酶的研究
李晓晖[1,2] 俞勇[3] 李会荣[3] 张琳[2] 任大明[1]
[1]复旦大学遗传工程国家重点实验室,上海200433 [2]上海水产大学食品学院,上海200090 [3]中国极地研究中心,上海200136
摘 要:
对北极海冰获得的14株低温微生物进行培养条件和胞外水解酶活力的研究，结果表明：（1）菌株最适生长温度均为15℃和20℃左右，最适pH在8．0左右，在酸性条件下几乎不生长。生长需要一定浓度的盐，在3％NaCl的培养基中能很好的生长，而在没有NaCl的培养基中不生长。（2）14株菌株能不同程度的分泌胞外水解酶。（3）菌株i429，i539在低温下产纤维素酶的活力最高，具有低温酶的特性。[著者文摘]
文章出处:
《上海水产大学学报》-2007年16卷6期 -531-536页
分 类 号:
文献标识码:
A
文章编号:
1004-7271（2007）06-0531-06
The studies of culture condition and extracellurar hydrolase of psychrophilic bacteria from Arctic sea ice
LI Xiao-hui, YU Yong , LI Hui-rong , ZHANG Lin, REN Da-ming （ 1. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China ; 2. College of Food Science and Technology, Shanghai Fisheries University, Shanghai 200090, China; 3. Polar Reasearch Institute of China, Shanghai 200136, China）
Abstract:
Studies was done on the culture condition and sreened of extracellurar hydrolase from 14 different cold adapted microorganisms. The result showed that optimal temperature of most of microorganisms was 15 ℃ and 20 ℃, The optimal pH at 8.0, hardly grown in acid condition, growth need the presence of NaCl, 14 strains produce extracellurar hydrolase with different activities, Strain i539 and i429 produced protease, cellulose and lipase, further results indicated that celluloses from i539 and i429 were low-tempature cellulose.[著者文摘]
Key words:
cold adapted microorganism ; screening; protease ; lipase ; amylase ; cellulose
基金资助:
国家自然科学基金（40376001）
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