学术
暗纹东方鲀几种组织中可培养细菌的组成分析
杨桂梅[1] 鲍宝龙[1] 李会荣[2] 唐文乔[1] 任大明[3]
[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 [2]中国极地研究中心国家海洋局极地科学重点实验室,上海200136 [3]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433
摘 要:
采用16S rDNA特征序列PCR-DGGE法，分析了不同饵料饲养的暗纹东方鲀的表皮、肠道和河鲀毒素累积组织（肝和卵巢）中可培养细菌的群落组成。根据分子系统发育分析，共鉴定出45种可培养细菌，在这些细菌中，以变形细菌的gamma亚群占多数，其它分别隶属于低GC含量革兰氏阳性菌和高GC含量革兰氏阳性菌。摄食不同饵料的暗纹东方鲍，其肠道、表皮或河鲀毒素累积组织中的细菌菌落组成是不同的，但不管是摄食人工配合饲料或天然饵料，在暗纹东方鲀的肠道、表皮或河鲀毒素累积组织中，均发现已有报道的可产河鲀毒素的细菌类群，表明饵料来源对暗纹东方鲀的河鲀毒素产生不是必需的。[著者文摘]
文章出处:
《上海水产大学学报》-2008年1期 -12-21页
分 类 号:
文献标识码:
A
文章编号:
1004-7271（2008）01-0012-10
Analysis on the composition of culturable bacterial communities in several tissues of puffer fish Takifugu obscurus
YANG Gui-mei , BAO Bao-long , LI Hui-rong, TANG Wen-qiao , REN Da-ming （ 1. Key laboratory for Aquatic Genetic Resources and Aquaeultureal Ecology Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China; 2. SOA Key Laboratory for Polar Science, Polar Research Institute of Chiua, Shanghai 200136, China; 3. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China）
Abstract:
Using 16S rDNA PCR denaturing gradient gel electrophoresis （PCR-DGGE） technology, we investigated the composition of culturable bacterial communities in the skin, intestines, and TTX-accumulating tissues （ ovary, liver） of the obscure striped puffer fish Takifugu obscurus fed natural or artificial diets. A total of 45 species of different culturable bacteria were isolated and classified according to phylogenetic analysis. Of these species, those belonging to the class γ-Proteobacteria dominated the microbial community in the puffer fish, while others belonged to the group of low G ＋ C Gram positive bacteria, and the group of high G ＋ C Gram positive bacteria. Large differences in the bacterial assemblage isolated from the intestines, TTX- producing organs and skins of fish fed natural and artificial diets were observed. No matter fed with natural or artificial diets, the tissue of intestine, TTX-accumulating organ, or skin, was found to contain bacteria from TTX-producing genera reported to-date. Puffer fish restricted to artificial diets from birth still had large assemblages of TTX-producing bacteria, indicating that food chain accumulation of TTX contributes little to the toxicity of these fish.[著者文摘]
Key words:
Takifugu obscurus; 16S rDNA; denaturing gradient gel electrophoresis （DGGE）; bacterial composition; TTX-producing bacteria
基金资助:
Fired： the Key Discipline funding Y1101, Key foundation program 06dj14003 from Shanghai Municipal Science Committee, China
更多>>免费文章
cqvip.com