学术
摘 要:
针对高压脉冲电场（PEF）对桦褐孔菌多糖提取率的影响进行了单因素和正交试验,并与热碱法、微波辅助提取法、超声波辅助提取法进行了对比试验。结果表明：当电场强度为30kV/cm、脉冲数为6、液料比为25mL/g、pH值为10时,桦褐孔菌多糖的提取率达到49.8%,是热碱提取法的1.67倍,是微波辅助提取法的1.12倍,多糖的纯度是超声辅助提取法的1.40倍。应用PEF技术提取桦褐孔菌中的多糖,提取时间短（12μs）,提取率高,提取物中杂质少,是一种有效的桦褐孔菌多糖提取方法。[著者文摘]
文章出处:
《农业机械学报》-2008年39卷2期 -89-92页
栏目信息:
文献标识码:
A
Study on Extraction of Polysaccharide from Inonotus Obliquus by High Intensity Pulsed Electric Fields
Yin Yongguang ,Cui Yanru, Wang Ting （Jilin University ）
Abstract:
Extraction of polysaccharide from Inonotus obliquus by way of applying high intensity pulsed electric fields （PEF） was studied. Through single-factor experiments and orthogonal experiment, the effect of PEF on polysaccharide extraction rate （ER） was discussed, and the comparison of alkali extraction method （TAEM）, microwave-assist extraction method （MAEM） and ultrasonic-assist extraction method （UAEM） has been done. A set of optimal parameters （PEF strength= 30 kV/cm, pulse number= 6, pH = 10, liquid/solid ratio = 25 mL/g） were tested and analyzed, at which the ER could be up to 49.8 %, 1.67 times of that by using TAEM, 1.12 times of MAEM, and the purity of polysaccharide was 1.40 times of UAEM. The experiments proved that PEF technique is an effective way to extract polysaccharide from the Inonotus obliquus, with higher extraction rate, shorter time （12μs）, and less impurities.[著者文摘]
Key words:
Inonotus obliquus, High intensity pulsed electric field, Extraction, Polysaccharide
基金资助:
吉林省科技发展计划资助项目（项目编号：20050202-3）
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