摘 要:
木质纤维素生产乙醇已成为世界各国研究开发的热点。但在酿酒酵母对木质纤维素稀酸水解产物的乙醇发酵中,对水解产物中的毒性物质非常敏感。菌种对水解液毒性物质耐受力相对较低是影响木质纤维素乙醇发酵工业化的主要因素之一。利用紫外线对酿酒酵母进行诱变,得到2株高效耐水解液中毒性物质突变株k和n。2株突变株发酵未脱毒的木屑稀酸水解产物的乙醇产量分别达到理论值的71.0%和61.3%,为进一步提高酿酒酵母耐毒性的研究和木质纤维素稀酸水解生产乙醇的工业化提供了基础。[著者文摘]
文章出处:
《可再生能源》-2007年3期,38 -31-33,38页
栏目信息:
文献标识码:
A
文章编号:
1671-5292(2007)03-0031-03
Mutagenesis and selection of high toxic tolerance Saccharomyces cerevisiae by the ultra-violet radiation
WANG Pu, TIAN Shen, WANG Dan, ZhANG Lan-bo, YANG Xiu-shan (Collegeof Life Science, Capital Normal University, Beijing 100037, China)
Abstract:
Ethanol is the new type of fuel which is researched by countries all over the world due to its high thermal efficiency, clean and nonpolluting characters as well as safety characters. Nowadays, toxic-tolerance ability of the yeasts was lower, which was one of the key factors restricting the development of fermentative ethanol producing industry. UV was used to irradiate Saccha- romyces cerevisiae in this experiment, and 2 mutants were gains, namely k and n.These two mutants would provide material for discuss the mechanism of the toxic tolerance of Saccharomyces cerevisiae and industry production of the bioethanol.[著者文摘]
Key words:
UV-mutagenesis; mutants; toxic tolerance; production of ethanol
基金资助:
国家“863”计划资助项目(2002AA514010和2001AA514024);北京市教委项目.

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