学术
摘 要:
根据PCV2Cap蛋白基因的序列设计引物，从1份患PMWS仔猪组织病料中扩增出Cap蛋白基因（702bp）。并利用基因工程技术将Cap蛋白基因克隆入腺病毒穿梭质粒pAdTrack-CMV中，将其与腺病毒骨架载体（pAdeasy-1）共转化大肠杆菌BJ5183，进行同源重组后转染293细胞，在293细胞内包装出重组腺病毒。通过免疫荧光、PCR和Westernblot检测证明PCV2Cap蛋白基因在293细胞内获得表达。[著者文摘]
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文章出处:
《中国预防兽医学报》-2008年30卷3期 -165-168页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1008-0589（2008）03-0165-04
Construction and expression of recombinant adenovirus carrying Cap gene of porcine circovirus type 2
OUYANG Su-zhen, ZHANG Fu-liang, WANG Shuang-shan, WANG Guo-dong, LIU Shu-mei （College of Biotechnology and Food Science, Anyang Institute of Technology, Anyang 455000, China）
Abstract:
The Cap protein gene of porcine circovitus （PCV） serotype 2 was amplified by PCR from a piglet with Posweaning multisystemic wasting syngrome （PMWS）. The gene was subcloned into the shuttle vector pAdTrack-CMV and transferred into E.coli B J5183 cells for homologous recombination with adenovirus backbone vector pAdeasy-1. Recombinant adenovirus genomic DNA was purified and transfected into 293 cells by lipofectamine. Adenovirus carrying Cap gene was screened and PCR confirmed. Expression of cap protein was detected by immunofluorescent assay and Westem blot.[著者文摘]
Key words:
porcine circovirus serotype 2 （PCV2）; Cap protein; adenovirus
基金资助:
河南省科技攻关项目（0624030004）
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