学术
新城疫病毒D90毒株对肺癌A549细胞生物学特性的研究
符芳[1] 刘春燕[3] 高瑞[1] 杨宝峰[2] 宋纯[2] 李曦[1]
[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室／猪病研究室,黑龙江哈尔滨150001 [2]哈尔滨医科大学,黑龙江哈尔滨150001 [3]黑龙江省科技成果转化中心,黑龙江哈尔滨150001
摘 要:
本试验采用细胞培养、吖啶橙染色、电镜、流式细胞仪等方法，研究了新城疫D90病毒株对肺癌A549细胞的体外杀伤作用。D90感染的细胞经吖啶橙染色后，可见到典型的细胞凋亡的形态学变化；电镜下可观察到凋亡小体，同时也观察到了典型的坏死细胞；流式细胞仪的分析结果表明，在24h的时间段，病毒的作用机制主要表现为凋亡，在48h时间段内，病毒的作用机制主要表现为坏死。人胎儿肝细胞单层培养物中接种D90后，未观察到细胞病变。结果表明，新城疫D90毒株作用于肺癌A549细胞的机制主要为凋亡与坏死相结合，因此它有期望成为一种有效的抗肿瘤生物制剂。[著者文摘]
文章出处:
《中国预防兽医学报》-2008年30卷3期,189 -179-182,189页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1008-0589（2008）03-0179-04
Anti-tumor effects of NDV D90 against tumor cells of A549 in vitro
FU Fang, LIU Chun-yan, GAO Rui, YANG Bao-feng, SONG Chun, LI Xi （ 1. Division of Swine Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Medicine Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China; 2. Harbin Medical University Harbin 150001, China; 3. Heilongjiang Scitech Achievements Transformation Center, Harbin 150001, China）
Abstract:
To study the antitumor effect of Newcastle disease virus （NDV）, lung cancer cells were infected by NDV D90 strain. The cells were stained with acridine orange and observed by flow cytometry and electron microscope techniques. Typical morphological changes of cell apoptosis were found in cells dyed by acridine orange. Apoptosis bodies and necrosis were observed through electron microscope. The results shown that D90 induced apoptosis at 24 h and caused necrosis at 48 h after lung cancer cells were infected. No obvious cell damages were observed in embryo liver cells. The antitumor effect of D90 strain could be a combination result of apoptosis and necrosis.[著者文摘]
Key words:
apoptosis; acridine orange; flow cytometry
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