学术
猪流行性腹泻病毒N蛋白单克隆抗体的制备与鉴定
冯力[1,2] 孙东波[1] 陈建飞[1] 时洪艳[1] 吴波平[3] 崔晓辰[1] 吕茂杰[1] 刘娣[2] 童光志[1]
[1]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室／猪传染病研究室,黑龙江哈尔滨150001 [3]福建省动物疫病预防控制中心,福建福州350003
摘 要:
以纯化的重组PEDVN蛋白为抗原免疫7周龄雌性BALB／c小鼠，利用淋巴细胞杂交瘤技术获得了7株稳定分泌抗重组N蛋白特异性单克隆抗体的杂交瘤细胞株，分别命名为9C1、7H8、3C10、6C4、10G9、2C10和483。7株杂交瘤细胞诱导小鼠产生的腹水抗体效价分别为1：3200、1：3200、1：6400、1：12800、1：25600、1：6400和1：12800。9C1、7H8、3C10、6C4、10G9和2C10免疫球蛋白类型均为IgM，轻链均为K链；483免疫球蛋白类型为IgG2a，轻链为K链。Westernblot试验结果显示，这7株单克隆抗体均能与天然的PEDVN蛋白发生反应。[著者文摘]
文章出处:
《中国预防兽医学报》-2008年30卷3期 -190-193页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1008-0589（2008）03-0190-04
Preparation and characterization of the monoclonal antibodies against nucleocapsid protein of PEDV
FENG Li, SUN Dong-bo, CHEN Jian-fei, SHI Hong-yan, WU Bo-ping, CUI Xiao-chen, LV Mao-jie, LIU Di, TONG Guang-zhi（ 1. Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China; 2. Post-doctoral Station of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 3. Fujian Center for Animal Disease Control and Prevention, Fuzhou 350003, China）
Abstract:
BALB/c mice were immunized with 50 μg of purified recombinant N protein of PEDV emulsified in Freund＇s adjuvant. Seven hybridoma cell lines 9C1, 7H8, 3C10, 6C4, 10G9, 2C10, and 4B3 that secreted monoclonal antibodies （McAbs） against recombinant N protein were established. The 9C1, 7H8, 3C10, 6CA, 10G9 and 2C10 were characterised by indirected ELISA as IgM subtype and κ chain, with ascetic titres of 1：3 200, 1：3 200, 1：6 400, 1 ： 12 800, 1：25 600, 1：6 400, respectively. The McAb 4B3 were IgG2a subtype with κ chain and had an ascetic titre of 1：12 800, Western blot showed that all seven McAbs were able to reacted with native N protein of PEDV.[著者文摘]
Key words:
porcine epidemic diarrhea virus; N protein; monoclonal antibody
基金资助:
黑龙江省博士后基金资助
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