学术
摘 要:
本研究以纯化的原核表达的猪轮状病毒VP7抗原表位区域为抗原，建立了检测猪轮状病毒抗体的间接ELISA诊断方法。特异性试验表明，该抗原与其他7种常见猪病病毒（TGEV、PEDV、CSFV、PCV2、PRRSV、PPV、PrV）的阳性血清不发生交叉反应，批内和批间重复性试验的变异系数均小于10％；对来自不同猪场的血清的检测结果表明，该ELISA方法与中和试验检测结果符合率达94．8％。本试验建立的ELISA诊断方法具有良好的重复性、敏感性和特异性，为PRV的快速诊断、免疫猪群抗体监测和轮状病毒流行病学调查提供了一种快速、简便的血清学诊断方法。[著者文摘]
文章出处:
《中国预防兽医学报》-2008年30卷3期 -233-237页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1008-0589（2008）03-0233-05
Development of an indirect ELISA for detection antibodies to porcine rotavirus using recombinant VP7 antigen
SHI Hong-yan, FENG Li, CHEN Jian-fei, SUN Dong-bo, CUI Xiao-chen, WANG Cheng-bao （National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China）
Abstract:
An indirect ELISA was developed to detect antibody to PRV using the recombinant protein derived from the epitopes region of VP7 protein. The recombinant VP7 protein antigen showed no cross-reaction with the positive sera of other seven swine diseases （TGE, PED, CSF, PCV, PRRS, PPV, PR）. The assay showed 94.8 % concordance with neutranization test. It was specific and sensitive, and could be used as a simple and rapid approach for monitoring of anti-PRV antibody and epidemiologic survey of PRV.[著者文摘]
Key words:
porcine rotavirus; recombinant VP7 protein antigen; indirect ELISA; diagnosis
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