学术
23个油橄榄品种的RAPD分析
邱源[1,2] 韩华柏[3] 李俊强[4] 侯春霞[1] 王永清[1]
[1]四川农业大学林学园艺学院,雅安625014 [2]四川省自贡市农业局,自贡643000 [3]四川省林业科学研究院,成都610081 [4]四川省宜宾职业技术学院,宜宾644003
摘 要:
采用RAPD技术对23个引种的油橄榄品种进行分类和鉴定研究。从80个10bp的随机引物中筛选出11个扩增效果较好的引物进行扩增,共产生127条带,其中78条为多态性带,占61.4%,平均每个引物扩增的DNA多态性带数为7.09条。4个品种具有特异的位点,可作为种质鉴定的依据。根据扩增结果构建反映品种间亲缘关系的UPGMA聚类图,23个品种可划分为2大类。[著者文摘]
文章出处:
《林业科学》-2008年44卷1期 -85-89页
文献标识码:
A
文章编号:
1001-7488（2008）01-0085-05
RAPD Analysis of 23 Olive （Olea europaea ） Cultivars
Qiu Yuan, Han Huabo, Li Junqiang, Hou Chunxia, Wang Yongqing（ 1. College of Forestry and Horticulture, Sichuan Agricultural University Ya＇an 625014; 2. Zigong Agricultural Bureau, Sichuan Province Zigong 643000 3. Sichuan Academy of Forestry Chengdu 610081 ; 4. Yibin Vocational and Technical College, Sichuan Province Yibin 644003）
Abstract:
Random amplified polymorphic DNA （RAPD） was used to study the classification and identification of 23 olive （Olea europaea） cuhivars. Eleven 10 bp primers selected from eighty arbitrary primers were applied for amplifying the olive DNA. Total 127 bands were produced, among which 78 bands （61.4%） were polymorphic. The average numbers of polymorphic DNA bands amplified by each primer were 7.09. There were specific bands in four cultivars which can be used in olive germplasm identification. A dendrogram showing genetic relationships was constructed through an unweighted pair-group method （UPGMA） based on the DNA polymorphism and the 23 cultivars were clustered into 2 main groups.[著者文摘]
Key words:
olive （Olea europaea）; RAPD analysis; cuhivar classification; genetic diversity
基金资助:
国家林业局四川森林生态与资源环境重点实验室资助.
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