摘 要:
对大孔树脂D001分离啤酒废酵母中的还原型谷胱甘肽进行了研究。其最佳试验条件为:pH4.5的0.02mol/L磷酸缓冲液平衡,pH7.5的0.02mol/L磷酸缓冲液洗脱,洗脱流速为2.0mL/min。在最佳分离条件下,大孔树脂D001分离GSH的平均收得率为20.03%,产品中GSH的含量为28.47%,产品颜色为白色。[著者文摘]
文章出处:
《现代食品科技》-2008年24卷2期 -131-133页
Modern Food Science and Technology
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1673-9078(2008)02-0131-03
相关文章:
Separation of Glutathione from Waste Beer Yeast by Macroporous Resin
HU Jing,QIU Yan-ling,MIAO Jing-feng,LIANG Liang(1.College of Bioengineering, Hubei University of Technology, Wuhan 430068, China) (2.Qilu pharmaceutical Co., LTD., Jinan 250000, China)
Abstract:
Macroporous resin D001 was adopted for separation of GSH from waste beer yeast, The result indicated that the optimum concentration of phosphate buffer, pH values for adsorption and elution and the elution rate were 0.02 mol/L, 4.5, 7.5, and 2.9 mL/min, respectively. Under those conditions, the purity of the crude white GSH was 28.47% analyzed by HPLC, and the average yield was 20.03%.[著者文摘]
收稿日期: 2007-11-05
基金资助:
湖北省教育厅重大项目资助(项目编号2003Z001)

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