学术
摘 要:
以中性红为光谱探针,采用荧光光谱和紫外-可见光谱法研究了葛根素与DNA的相互作用,考察了离子强度和I^-离子浓度对葛根素与DNA作用的影响。结果表明,在生理条件下（pH 7.4）,葛根素通过沟槽结合方式与DNA发生作用,DNA对葛根素荧光猝灭属于静态猝灭,测得其结合常数为4.81×104L/mol。[著者文摘]
文章出处:
《分析试验室》-2008年27卷3期 -1-4页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1000-0720（2008）03-001-04
Studies on the interaction between puerarin and DNA by spectrometry
ZHANG Guo-wen , GUO Jin-bao , QUE Qing-min ,PAN Jun-hui （Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047）
Abstract:
The interaction between puerarin and DNA was studied by fluorescence and absorption spectrometric methods using neutral red as a spectral probe. Effect of ionic strength and I^- concentration on the binding of puerarin with DNA were investigated. It is found that puerarin can react with DNA under physiological condition （pH 7.4）. The major mode of recognition is groove binding between puerarin and DNA. The quenching belonged to static fluorescence quenching. The binding constant for puerarin with DNA was 4.81×104 L/mol.[著者文摘]
Key words:
Puerarin; ct-DNA; Fluorescence spectra; Interaction
基金资助:
教育部长江学者和创新团队发展计划（IRT0540）和江西省教育厅基金（赣教技字[2005]-71号）项目资助
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