学术
摘 要:
运用流式细胞仪测定了双壳纲8种贝类的细胞核DNA含量。结果显示，测定贝类的DNA含量分别为（pg）：太平洋牡蛎，2．06±0．03；东方海笋，2．11±0．07；近江牡蛎，2．18±0．13；结蚶，2．59±0．10；青蚶，3．08±0．23；砂海螂，3．24±0．28；缢蛏，3．85±0．10；日本镜蛤，5．00±0．20。双因素方差分析表明，同种贝类个体间DNA含量的差异不显著（P〉0．05），仅占总差异的0．12％；而种间差异极为显著（P〈0．01），比例高达97．45％。本实验所测8种贝类的DNA含量与其进化地位间未呈现出明显的相关性。[著者文摘]
文章出处:
《中国海洋大学学报》-2007年37卷4期 -576-580页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1672-5174（2007）04-576-05
Quantitative Analysis of DNA Contents of Eight Species Belonging to Bivalvia and Primary Discussion on the Relationship Between C-Values and Evolutionary Degrees
WANG Zhao-Ping, GAO Ling-Yun, YU Rui-Hai, LI Jun （The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003,China）
Abstract:
The nuclear DNA contents of eight Bivalvia species were quantified using flow cytometry. The mean C-values （in picograms, pg） of the species were as follows, respectively. Crassostrea gigas, 2.06 ± 0.03; Pholasorientalis, 2.11 ± 0.07; Crassostrea rivularis, 2.18 ± 0.13; Tegillarca nodifera, 2.59 ± 0.10; Barbatia virescens, 3.08 ± 0.23 ; Mya arenaria, 3.24 ± 0.28 ; Sinonovacula constricta, 3.85 ± 0.10 ; and Dosinia japonica, 5.00± 0.20. Almost no difference was detected at individual levels （p 〉0.05）, while the interspecies difference was very significant （p 〈 0.01） according to the two-way ANOVA. A high percentage of the total variation was obtained at the interspecies level （97.45 % ） with only 0.12 % at the intraspecies level. Evolutionary degree of these species could not be resolved clearly on the bass of their DNA contents merely.[著者文摘]
Key words:
bivalvia; DNA content ; evolutionary degree
基金资助:
中国海洋大学海水养殖教育部重点实验室开放课题资助
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