学术
内皮型一氧化氮合酶基因A-922G多态性与缺血性脑卒中易感性的关联研究
程锦泉[1] 刘建平[1] 张仁利[1] 庾蕾[1] 彭绩[2] 韩胜红[3] 聂绍发[3]
[1]深圳市疾病预防控制中心,深圳518020 [2]深圳市慢性病防治院,深圳518020 [3]华中科技大学同济医学院,深圳518020
摘 要:
目的探讨内皮型一氧化氮合酶（eNOS）基因5’侧翼区-922A/G多态性与缺血性脑卒中关系。方法采用1∶1配比病例-对照研究设计,选择深圳市两家综合性医院的309例缺血性脑卒中患者为病例组,同时选择年龄、性别匹配的309例健康者作为对照。采用TaqmanMGB探针荧光定量PCR技术检测-922A/G变异,同时按照流行病学方法设计调查表进行问卷调查。结果-922A/G变异基因型（AA、AG、GG）频率缺血性脑族中组分别为78.96%、17.80%和3.24%;对照组基因型分布频率分别为85.11%、13.59%和1.29%。病例组的G等位基因频率（12.14%）明显高于对照组（8.09%）（P=0.018）;携带至少一个G等位基因的基因型个体患缺血性脑卒中的风险为AA基因型的1.523倍（95%CI：1.005-2.309）;条件logistic回归分析显示,在调整吸烟、饮酒、腰臀比、体重指数和高血压病史等因素的影响后,A-922G变异对缺血性脑卒中仍具有影响,OR为2.156,95%CI：1.081-4.299。结论eNOS基因-922A/G多表性可能与缺血性脑卒中的易感性有关联。[著者文摘]
文章出处:
《卫生研究》-2008年37卷1期,7 -1-3,7页
栏目信息:
文献标识码:
A
文章编号:
1000-8020（2008）01-0001-04
Study on A-922G genetic polymorphism of endothelial nitric synthase associated ischemic stroke
CHENG Jinquan, LIU Jianping, ZHANG Renli, YU Lei, et al. （ Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China）
Abstract:
Objective To explore the relationship between A-922G genetic polymorphism in the 5＇flanking region of the endothelial nitric oxide synthase （eNOS） gene and ischemic stroke. Methods A 1 ： 1 matched case-control study was conducted. 309 patients suffered from ischemic stroke were selected from two general hospitals in Shenzhen. The controls were selected by the same gender and ethnic group, and each pair＇s ages were permitted of differ within 5 years, eNOS A- 922G genotypes were determined by using the real-time PCR technology for SNP genotyping with Taqman MGB probes. The cases and controls were interviewed by using the same questionna!re. Results The genotype frequencies （AA, AG and GG） of eNOS A-922G were 78.96%, 17.80, and 3.24% in patients, and 85.11%, 13.59, and 1.29% in controls. The allele frequencies of eNOS - 922G in case group （12.14%） were higher than those in the controls （8.09%） （P = 0.018）. OR value of ischemic stroke for subjects with at least one G allele was 1.523 （ OR 95% CI 1.005 - 2.309）. Results of conditional logistic regression demonstrated that eNOS A-922G genetic polymorphism also had significant effects on ischemic stroke （ OR = 2. 156, 95 % CI 1. 081 - 4. 299） after adjusting smoking, alcohol drinking, waist-to-hipratio, body mass index and history of hypertension, et al. Conclusion It was suggested that A-922G gene polymorphism of eNOS gene could associate with the higher risk of ischemic stroke susceptibility.[著者文摘]
Key words:
ischemic stroke, nitric oxide synthase, genetic polymorphism
基金资助:
国家自然科学基金资助项目（No.30771852）;深圳市科技局项目（No.200304208）
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