学术
摘 要:
目的从亚基基因表达方面探讨NMDA受体在微波辐照所致的学习记忆功能障碍中的作用。方法采用平均功率密度为65mW/cm^2的微波全身一次辐照大鼠20min（SAR12.0W/kg）,在辐照后0h、3h、12h、24h和3d时相点,大鼠腹腔麻醉断头,分离海马,用RT-PCR技术分别检测NMDA受体功能亚基NR1和调节亚基NR2A、NR2B、NR2C、NR2D在基因表达水平上的变化。结果微波辐照后大鼠海马脑区NMDA受体功能亚基NR1和调节亚基NR2A、NR2C、NR2D基因表达异常。表现为功能亚基NR1mRNA表达在辐照后3h、24h、3d时相分别比对照组下降21.1%（P〈0.05）、14.2%（P〈0.05）、30.3%（P〈0.01）;辐照后0h、3h、12h,NR2AmRNA表达较对照组分别降低37.0%（P〈0.01）、35.9%（P〈0.05）、35.3%（P〈0.05）;NR2BmRNA表达与对照组比无统计学差异;辐照后0h和24h,NR2CmRNA表达较对照组分别降低24.3%（P〈0.05）和27.1%（P〈0.05）;辐照后0h、12h、24h、3d,NR2DmRNA表达较对照组分别升高67.9%（P〈0.05）、45.7%（P〈0.05）、49.7%（P〈0.01）、75.4%（P〈0.01）。结论微波辐照后大鼠海马脑区NMDA受体结构组成发生改变,NMDA受体自身的复杂调节功能下降,从而影响受体介导的LTP的产生。[著者文摘]
文章出处:
《卫生研究》-2008年37卷1期 -25-28页
栏目信息:
文献标识码:
A
文章编号:
1000-8020（2008）01-0025-04
Effects of microwave irradiation on NMDA receptor subunits mRNA expressions in rat hippocampus
ZHANG Yanwen, YU Zhengping, XIE Yan, FANG Qiang, et al. （The Key Laboratory of Ministry of Education and PLA for EMR Medical Protection, the Third Military Medical University, Chongqing 400038, China）
Abstract:
Objective To study the role of NMDA receptor in learning and memory deficit caused by microwave irradiation. Methods At the time of Oh, 3h, 12h, 24h, 3d after microwave irradiation of 65mW/cm^2 exposure for 20min （SAR 12.0 W/kg）, rats were decapitated under deep anesthesia, the hippocampus was quickly removed, then expressions of NR1 , NR2A, NR2B, NR2C, NR2D subunits of NMDA receptor were measured by RT-PCR. Results Compared with control, the mRNA expressions of NR1 decreased in 21.1% （ P 〈 0.05）, and in 14.2 % （ P 〈 0.05）, and in 30.3 % （ P 〈 0.01 ） respectively at timepoints of 3h, 24h, 3d after microwave exposure. At timepoints of Oh, 3h, 12h after microwave exposure, the mRNA expressions of NR2A decreased in 37.0% （ P 〈 0.01）, and in 35.9% （ P 〈 0.05）, and in 35.3% （P 〈 0.05） respectively. The mRNA expressions of NR2B did not statistically change after microwave exposure. The mRNA expressions of NR2C decreased in 24.3 % （ P 〈 0.05）, and in 27.1% （ P 〈 0.05） respectively at timepoints of Oh, 24h after microwave exposure. At timepoints of Oh, 12h, 24h, 3d after microwave exposure, the mRNA expressions of N R2D increased in 67.9%（P〈0.05）, and in 45.7%（P〈0.05）, and in 49.7%（P〈0.01）, and in 75.4%（P〈 0.01 ） respectively. Conclusion The results indicated that the composition of subunits comprising NMDA receptor could be altered and autoregulation of NMDA receptor in the rat hippocampus exposed by microwave could decrease. These changes could be correlated with impaired LTP induced by microwave irradiation.[著者文摘]
Key words:
microwave, hippocampus, NMDA receptor
基金资助:
国家自然科学基金资助项目（No.30270349）
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