学术
瘦素抵抗肥胖大鼠脂肪组织SOCS-3、PPARγ及ACO mRNA水平的探讨
刘莉[1] 顾海伦[2] 赵越[1] 于飞[1] 任亚浩[1] 马爽[1] 陈丽丽[1] 杨军[1]
[1]中国医科大学公共卫生学院营养与食品卫生教研室,沈阳110001 [2]中国医科大学附属盛京医院骨外二科,沈阳110001
摘 要:
目的观察高脂饮食诱导的肥胖瘦素抵抗大鼠细胞因子信号转导抑制因子-3（SOCS-3）、过氧化物酶体增殖物激活受体γ（PPARγ）及乙酰辅酶A氧化酶（ACO）mRNA表达水平的变化。方法30只Wistar雄性大鼠,6只为对照组喂饲基础饲料,24只为高脂组喂饲高脂饲料,第8周末按体重增量从高脂组中筛选出8只大鼠作为肥胖组,测定对照组和肥胖组大鼠血清瘦素,附睾脂肪组织中SOCS-3,PPARγ以及ACO mRNA表达。结果肥胖组大鼠体重以及血清瘦素水平均显著高于对照组（P〈0.05）;肥胖组脂肪组织SOCS-3、PPARγ mRNA水平显著高于对照组（P〈0.05）,而ACO mRNA则显著低于对照组（P〈0.05）。结论高脂饮食诱导的肥胖大鼠存在瘦素信号转导通路的抑制,脂肪酸氧化能力降低,脂肪合成能力增强。[著者文摘]
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文章出处:
《卫生研究》-2008年37卷1期 -40-42页
栏目信息:
文献标识码:
A
文章编号:
1000-8020（2008）01-0040-03
Study on SOCS-3, PPARγ and ACO mRNA expression of adipose tissues in obese rats with leptin resistance
LIU Li, GU Hailun, ZHAO Yue, YU Fei, et al.（ School of Public Health, China Medical University, Shenyang 110001, China）
Abstract:
Objective To study the difference of socs-3, PPART and ACO mRNA expression in obese rats with leptin resistance induced by high-fat diet. Methods 6 Wistar male rats were fed with basic diet as control, and 24 rats were fed with high-fat diet as high-fat diet group. At the end of the 8th week, 8 obese rats were selected by body weight from highfat diet group as obese group. Serum leptin, socs-3, PPART and ACO mRNA were detected. Results In compare with control group, body weight, serum leptin, socs-3 and PPARγ mRNA expressions of epididymal adipose tissues were significantly increased in obese group, while ACO mRNA were markedly decreased. Conclusion Obese rats induced by high-fat diet showed the suppression of leptin signaling transduction pathway, decreases in fatty acid oxidation and increases in fat synthesis.[著者文摘]
Key words:
leptin resistance, obese rats, suppressor of cytokine signaling 3, peroxisome pruliferators-activated receptor-γ, acyl-CoA oxidase
基金资助:
国家自然科学基金资助项目（No.30500409）
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