学术
摘 要:
目的研究基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）用于检测鉴定细菌耐药性的可行性。方法对临床分离的耐药谱明确的8属11株细菌及相应的标准菌株,经相同条件培养后,应用MALDI-TOF-MS进行检测,并分析其耐药株和标准株质谱图的差异。另外,对临床分离的30株金黄色葡萄球菌同时进行药物敏感性实验和MALDI-TOF-MS检测。结果应用MALDI-TOF-MS检测细菌耐药性时,对葡萄球菌检测结果较好,尤其是耐甲氧西林金黄色葡萄球菌,而对其它细菌耐药性检测效果较差。结论MALDI-TOF-MS检测细菌耐药性适用于部分细菌。[著者文摘]
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文章出处:
《卫生研究》-2008年37卷1期 -82-85页
栏目信息:
文献标识码:
B
文章编号:
1000-8020（2008）01-0082-04
Primary research on detecting bacteria drug resistance by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry
SHI Guiying, SUN Zongke, CHEN Xiping（ Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China）
Abstract:
Objective To study the feasibility of bacteria drug resistance determinated by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）. Methods 11 strains of 8 genera in drug resistant-bacteria separated from clinical specimens and the corresponding standard strains were detected by MALDI-TOF- MS, and the differences of the mass spectrums were analyzed. At the same time, 30 strains of Staphylococcus aureus separated from clinical specimens were detected by MALDI-TOF-MS and their antimicrobial susceptibility were tested. All bacteria were cultured under the same conditions. Results When drug-resistant bacteria determinated by MALDI-TOF- MS, it was efficient for Staphylococcus, especially the methicillin-resistant Staphylococcus aureus, but it was not efficient for other genera. Conclusion MALDI-TOF-MS can be used to detect drug resistance of some bacteria.[著者文摘]
Key words:
matrix-assisted laxer desorption ionization time-of-flight mass spectrometry, drug-resistant bacteria, mass spectrum, bacteria
基金资助:
国家自然科学基金资助项目（No.30371194）
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