学术
桑叶多糖的分离及红外光谱和气相色谱分析
张剑韵[1] 包立军[1] 梁进[1] 黄龙全[2]
[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学茶与食品科技学院,合肥230036
摘 要:
多糖是桑叶中的重要功能性成分之一。采用水煮提取、乙醇分级沉淀和Sephadex G-100凝胶层析的方法，从桑叶中分离、纯化出3神多糖组分：MPSⅠ、MPSⅡ和MPSⅢ。经红外光谱分析，MPSⅠ不同于MPSⅡ和MPSⅢ，表现出长分子链、β-糖苷键构型和含酰胺基团等特征。气相色谱分析结果显示，L-鼠李糖、D-葡萄糖和D-半乳糖是3种桑叶多糖的共同成分，其中D-葡萄糖和D-半乳糖的构成比例较大，3种桑叶多糖的单糖构成也存在明显差异。[著者文摘]
文章出处:
《蚕业科学》-2007年33卷4期 -549-552页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0257-4799（2007）04-0549-04
Infrared Spectrum and Gas Chromatogram Analysis of the Polysaccharides Separation From Mulberry Leaves
ZHANG Jian-Yun, BAO Li-Jun, LIANG Jin, HUANG Long-Quan （1 College of Life Science, Anhui Agricultural University, Hefei 230036, China; 2 College of Tea ＆ Food Science, Anhui Agricultural University, Hefei 230036, China）
Abstract:
Three kinds of polysaccharides （MPSⅠ , MPSⅡ, MPSⅢ） were separated from the water extract of mulberry leaves, by fractional precipitation with ethanol concentrations at 30%, 50% and 80% respectively, and further purified by sephadex G-100 gel column chromatography. Compared with the other two polysaccharides, MPSⅠ charaeterized as β-glycosidic bond, a longer molecule chain and a kind of glycosaminoglycan by infrared spectrum analysis. Gas chromatogram results showed that L-rhamnose, D-glucose and D-galactose are the common components, and D-gLucose and D-galactose are the major monosaccharide in the mulberry polysaccharides. Monosaccharide constitution also showed obvious difference among the three kinds of mulberry polysaccharide.[著者文摘]
Key words:
Polysaccharide in mulberry leaves; Separation; Infrared spectrum; Gas chromatogram
基金资助:
安徽省教育厅自然科学研究项目（编号2003KJ126）;安徽省教育厅自然科学重点科研项目（编号2005KJ393ZD）.
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