学术
摘 要:
在对家蚕蛹cDNA文库的测序中发现了脂多糖诱导肿瘤坏死因子α的转录激活因子（lipopolysaccharide-induced tumor necrosis factor-α factor, LITAF，LITAF）的EST序列（GenBank登录号AADK01016184）。经过比对发现BmLITAF全长为981bp，由97bp的5′端非翻译区序列（5′UTR）、390bp的开放读码框（ORF）和494bp的3′端非翻译区序列（3′UTR）组成。用电子克隆方法对BmLITAF进行基因结构分析发现，此基因由3个外显子和2个内含子组成，编码129个氨基酸。对BmLITAF蛋白进行疏水性、跨膜结构和信号肽分析的结果显示，BmLITAF具有跨膜结构域。根据BmLITAF的电子克隆序列由家蚕基因组PCR扩增获得BmLITAF基因，将其克隆到pGEM-T-easy载体中，测序验证与电子克隆结果一致。[著者文摘]
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文章出处:
《蚕业科学》-2007年33卷4期 -562-567页
栏目信息:
文献标识码:
A
文章编号:
0257-4799（2007）04-0562-06
In Silico Cloning and Sequence Analysis of a Novel Gene, BmLITAF, From Silkworm, Bombyx mori
LIU Yu-Sheng, CHEN Jian, NIE Zuo-Ming ,LU Zheng-Bing ,WANG Dan ,WU Xiang-Fu ,ZHANG Yao-Zhou （Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China）
Abstract:
The EST of lipopolysaccharide-induced tumor necrosis factor-co factor （LITAF, GenBank accession no. AADK01016184） was found through searching cDNA bank of silkworm pupae. The full-length of the gene is 981 bp, which contains 5＇ UTR （97 bp） ,ORF （390 bp） and 3＇ UTR （494 bp）. Genome structure of BmLITAF is composed of three exons and two introns and encodes 129 amino acids. Moreover, there is an transmembrane structure domain in BmLITAF. According to the in silico clone sequence of BmLITAF, the gene BmLITAF got from genome of Bombyx mori by PCR and cloned to pGEM-T-easy vector. The sequencing result showed that the clone sequence was identical with in silico clone sequence.[著者文摘]
Key words:
Bombyx mori; BmLITAF; In silico cloning; Sequence analysis
基金资助:
国家高技术研究计划“863”项目（编号2005AA206120）;国家重点基础研究发展计划“973”项目（编号2005CB121-000）;浙江自然科学基金项目（编号Z204267）.
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