学术
家蚕丝腺Matchmaker cDNA文库的构建和3种蚕丝蛋白基因转录因子编码区的克隆
王兴科[1] 汪伟[1] 汪生鹏[1,2] 郭锡杰[1,2]
[1]江苏科技大学生物与环境工程学院,镇江212018 [2]中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江212018
摘 要:
采用Clontech公司SMART^TM技术，分别构建了家蚕4龄眠蚕后部丝腺（PSGO）以及5龄第3天后部丝腺（PSG3）、中部丝腺（MSG3）和脂肪体（FAT3）的Matchmaker cDNA文库，用A3基因引物对几个文库的质量进行了检测；从cDNA文库和家蚕基因组中克隆了FMBP-1、POU-MI和BmFkh3种在蚕丝蛋白基因表达调控中起重要作用的转录因子编码区，并对其序列进行了分析。结果表明，所建cDNA文库能够用于利用酵母的杂交系统开展蚕丝蛋白基因的表达调控研究。[著者文摘]
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文章出处:
《蚕业科学》-2007年33卷4期 -574-580页
栏目信息:
文献标识码:
A
文章编号:
0257-4799（2007）04-0574-07
Constuction of Matchmaker cDNA Libraries of Silk Gland From Bombyx mori and Cloning of 3 trans Factors Involved in Silk Genes＇ Controlling
WANG Xing-Ke, WANG Wei, WANG Sheng-Peng, GUO Xi-Jie （ 1 College of Biotechnology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212018, China; 2 The Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu 212018, China）
Abstract:
Using SMART^TM cDNA library construction technology from Clontech biocompany, we have constructed 4 Matchmaker cDNA libraries for posterior silk gland of 4^th moulting larvae, posterior silk gland, middle silk gland and fat body of larvae at 3^rd day of 5^th instar, respectively. Quality of the libraries was checked using A3 gene specific primers. CDSs of 3 main trans factors involved in controlling silk genes＇ expression were cloned and sequenced from the libraries and silkworm genomic DNA. Results showed that these libraries can be used in the study of silk genes＇ controlling using yeast hybrid technology.[著者文摘]
Key words:
Bombyx mori; Silk gland; Matchmaker cDNA library; trans factor
基金资助:
国家重点基础研究发展计划“973”项目（编号2005CB-121004）;国家自然科学基金项目（编号30771631）;江苏省自然科学基金项目（编号BK2007099）;江苏科技大学人才资助项目（编号2006CY122J）.
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