学术
家蚕对浓核病毒中国（镇江）株抵抗性机制的初步研究
裘智勇[1,2] 李木旺[1] 覃光星[1] 刘挺[1] 沈兴家[1] 郭锡杰[1,2]
[1]中国农业科学院蚕业研究所,农业部家蚕生物技术重点开放实验室,镇江212018 [2]江苏科技大学,镇江212003
摘 要:
通过生物测定和地高辛（DIG）标记的酶联免疫吸附剂测定（ELISA），初步分析了家蚕对浓核病毒中国（镇）株（Bombyx mori densovirus，BmDNV-z）的抵抗性机制。试验表明：供试家蚕品种的消化液、血液中均不存在对BmDNV-z侵染性有影响的蛋白因子；抵抗性家蚕品种的中肠组织蛋白中不存在BmDNV-z的病毒受体蛋白，而感受性家蚕品种的中肠组织蛋白中可能存在对BmDNV-z侵染性有影响的蛋白因子，即感受性家蚕品种的中肠组织中可能存在BmDNV-z的病毒受体蛋白因子，且BmDNV-z与感受性家蚕品种中肠组织蛋白的结合具有组织特异性。[著者文摘]
文章出处:
《蚕业科学》-2007年33卷4期 -596-601页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0257-4799（2007）04-0596-06
Primary Studies on Mechanism of Silkworm （ Bombyx mori） Resistance to Densovirus China （Zhenjiang） Strain
QIU Zhi-Yong, LI Mu-Wang, QIN Guang-Xing, LIU Ting, SHEN Xing-Jia, GUO Xi-Jie （1 The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang Jiangsu 212018, China; 2 Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212003, China）
Abstract:
To investigate the mechanism of silkworm resistance to Bombyx moil densovirus （BmDNV-z）, the infectivity of virus to silkworm after incubated with extract of different tissues of silkworm of susceptible and non-susceptible varieties was tested by bio-assay, and the combination activity of BmDNV-z with proteins from the extracts was measured by DIG-labeled ELISA. The results indicated that there were no protein factors affecting the invasion of BmDNV-z in the digestive juice and the blood of the silkworm. Some protein factors which enhanced the invasion of BmDNV-z existed in the midgut of susceptible variety. We speculated that protein factor as virus receptor may exist in the midgut of susceptible variety, but not in the non-susceptible variety. The combination activity was tissue-specific to the midgut tissue of susceptible variety.[著者文摘]
Key words:
Bombyx mori; Midgut tissue; Resistance; Densovirus
基金资助:
国家重点基础研究发展计划“973”项目（编号2005CB-121004）;国家科技支撑计划项目（编号2006BAD06B03）;国家自然科学基金项目（编号30400327）.
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