学术
蓖麻蚕核型多角体病毒p6．9基因的克隆及序列分析
费建明[1,2] 李兵[2] 沈卫德[2]
[1]浙江省湖州市农业科学研究院,湖州313000 [2]苏州大学生命科学学院,苏州215123
摘 要:
基于丰富蓖麻蚕核型多角体病毒（Philosamia cynthia ricini nucleopolyhedrovirus, PcrNPV）分子信息的目的，对PcrNPV DNA部分片段进行了测序分析，获得1个DNA结合蛋白基因p6．9的完整序列。该基因的读码框由240个核苷酸组成，编码79个氨基酸，分子质量约为9．8kD，转录起始位点ATG侧翼序列符合Kozak规则，其上游34bp处具有晚期基因转录的保守起始序列taag，表明该基因为晚期表达基因。将PcrNPV与12种昆虫核型多角体病毒P6．9蛋白氨基酸序列作同源性比较的结果显示：PcrNPV P6．9蛋白氨基酸序列与家蚕（Bombyx mori）NPV P6．9的同源性为59％，与柞蚕（Antheraea pernyi）NPV的同源性为100％，表明PcrNPV与ApNPV的亲缘关系很近。[著者文摘]
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文章出处:
《蚕业科学》-2007年33卷4期 -610-613页
栏目信息:
文献标识码:
A
文章编号:
0257-4799（2007）04-0610-04
Cloning and Sequence Analysis of p6. 9 Gene of Philosamia cynthia ricini Nucleopolyhedrovirus
FEI Jian-Ming, LI Bing, SHEN Wei-De （ 1 Academy of Agricultural Sciences of Huzhou City, Huzhou Zhejiang 313000, China; 2 School of Life Science, Soochow University, Suzhou Jiangsu 215123, China）
Abstract:
To obtain more molecular information of Philosamia cynthia ricini nucleopolyhedrovirus （ PcrNPV）, some fragments of PcrNPV DNA were sequenced and analyzed. The p 6.9 gene was found, which is 240 base pair long and encodes a protein of 79 amino acids with a predicted molecular weight of 9.8 kD. A late baculoviral transcription initation motif （taag） was located at 34 bp upstream of the putative translation start Site （atg）, suggesting that the p 6.9 gene may be a late gene. The nucleotide sequence analysis of p 6.9 between PcrNPV and 12 insect NPVs indicated that the PcrNPV P6.9 has 59% and 100% identities with that of Bombyx mori NPV（BmNPV） and Antheraea pernyi NPV（ApNPV）, respectively.[著者文摘]
Key words:
Philosamia cynthia ricini nucleopolyhedrovirus; p 6.9 gene; Sequence analysis
基金资助:
国家重点基础研究发展计划“973”项目（编号2005CB-2121005）.
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