学术
野桑蚕和不同家蚕品系幼虫体内1-脱氧野尻霉素含量测定
沈以红[1] 朱见[2] 代方银[1] 李竞[2] 向仲怀[1]
[1]农业部蚕桑学重点开放实验室,重庆400716 [2]西南大学药学院,重庆400716
摘 要:
采用反相高效液相色谱（RP-HPLC）-紫外检测法测定了野桑蚕和不同品系家蚕体内的1-脱氧野尻霉素（1-DNJ）含量，并调查了家蚕5龄幼虫不同组织不同生长时期1-DNJ含量的变化。野桑蚕与家蚕、家蚕不同品系间的1-DNJ含量存在极显著差异：野桑蚕全蚕粉中1-DNJ含量最高，质量分数平均为0．4278％；家蚕以黄血蚕最低，平均0．2205％。家蚕5龄幼虫不同组织的1-DNJ含量也存在明显差异：家蚕血干粉中的1-DNJ含量最高，质量分数达0．8487％；其次是中肠、体壁和脂肪体组织，分别为0．5122％、0．4722％、0．3085％；而在丝腺中几乎检测不出1-DNJ。家蚕5龄幼虫体内1-DNJ含量随发育时期呈明显变化，5龄第4天含量最高，达0．329％，第5天以后迅速下降。[著者文摘]
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文章出处:
《蚕业科学》-2007年33卷4期 -674-677页
栏目信息:
文献标识码:
A
文章编号:
0257-4799（2007）04-0674-04
Investigation of 1-DNJ Contents in the Larvae of Bombyx mori and Bombyx mandarina
SHEN Yi-Hong,ZHU Jian, DAI Fang-Ying, LI Jing, XIANG Zhong-Huai（ 1 The Key Sericulture Laboratory of Agricultural Ministry, Chongqing 400716, China; 2 Pharmaceutical College of Southwest University, Chongqing 400716, China）
Abstract:
In this paper, the 1-deoxynojirimycin（1-DNJ） contents in the 5th instar larvae at different developmental stages and tissues of Bombyx mori and Bombyx mandarina were investigated by Reverse-phase High-performance Liquid Chromatography. The results showed that there were obvious 1-DNJ changes in larvae between Bombyx mori and Bombyx mandarina, as well as among various Bombyx mori strains. The highest 1-DNJ content was found in Bombyx mandarina larvae and the average is 0. 427 B%. The domesticated silkworm with yellow blood has the lowest 1-DNJ content, and the average level is about 0. 220 5%. The 1-DNJ content of translucent silkworm larvae is extremely higher than that of the normal one, and the normal is higher than that with yellow blood. The differences of 1-DNJ content among different tissues are obvious. The highest is in the blood, the next is in the midgut, fat body, and skin, in order. The silkgland almost has no 1-DNJ. The 1-DNJ content of domesticated silkworm larvae changes significantly with developmental stages. It reached a peak of 0. 329% at the 4th day of the 5th instar and then decreased quickly.[著者文摘]
Key words:
Bombyx mori; Bombyx mandarina; 1-Deoxynojirimycin; Reverse-phase high-performance liquid chromatography（RP-HPLC）
基金资助:
重庆市院士基金课题（编号9215）.
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