家蚕微孢子虫（Nosema bombycis）侵染家蚕丝腺组织后的免疫胶体金标记观察
吴正理[1] 李艳红[1] 党晓群[1] 谭小辉[1] 潘国庆[1] 周泽扬[1,2] 向仲怀[1]
[1]农业部蚕桑学重点开放实验室,重庆400716 [2]重庆师范大学生命科学学院,重庆400047
摘 要:
为了探讨家蚕微孢子虫（Nosema bombycis）功能基因组研究方法和手段，利用免疫胶体金标记电镜技术。对被感染的家蚕丝腺组织中家蚕微孢子虫的蛋白质分泌状况进行了观察。结果显示，有大量的胶体金颗粒存在于家蚕微孢子虫寄生位点的宿主组织内，说明了家蚕微孢子虫寄生位点的宿主组织内存在大量的孢子蛋白质，证实了家蚕微孢子虫在寄生过程中将许多孢子蛋白质分泌到宿主体内。初步探讨了家蚕微孢子虫与宿主家蚕之间的互作关系。[著者文摘]
文章出处:
《蚕业科学》-2007年33卷4期 -682-685页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0257-4799（2007）04-0682-04
Distribution of Secreted Proteins of Nosema bombycis in Infected Silk Gland by Colloid Gold Labelling and Immunoelectron Microscope Technique
WU Zheng-Li, LI Yan-Hong, DANG Xiao-Qun, TAN Xiao-Hui, PAN Guo-Qing, ZHOU Ze-Yang, XIANG Zhong-Huai（1 The Key Sericultural Laboratory of Agricultural Ministry, Chongqing 400716, China; 2 College of Life Sciences, Chongqing Normal University, Chongqing 400047, China）
Abstract:
To explore the method of studying function genome of Nosema bombycis,colloid gold labelling and immunoelectron microscopy technique was used to investigate the distribution of secreted proteins of Nosema bombycis in infected silk gland. The results showed that there were a lot of gold particles at the spore parasitized site, demonstrating that there existed many spore proteins in the parasitized site. It suggested that many spore proteins secreted in the host cell. The preliminary interaction between N. bombycis and its host silkworm was investigated.[著者文摘]
Key words:
Nosema bombycis; Ultrastructure; Colloid gold labelling technique; Morphology
基金资助:
国家重点基础研究发展计划“973”项目（编号2005CB-121000）;国家自然科学基金项目（编号30600452）;重庆市重大科技攻关项目（编号2006AA5019）;国家茧丝办发展风险基金项目（编号M012005-000Y-00070）.
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