学术
摘 要:
从内蒙古四子王旗燕麦根际土壤分离获得5株具有解磷能力的细菌，从中筛选出2个解磷能力相对较强的菌株，对其形态学特征进行了观察，并比较了不同碳源、盐浓度、pH等环境条件对其生长的影响。结果表明，2个菌株特别是YM-2具有较强的耐盐性；适宜生长的介质pH为中性偏碱性；葡萄糖是最好的碳源。随着有机磷细菌的生长繁殖，介质pH会逐渐降低，泌酸以及耐盐等特性对改善种植于盐渍化土壤的燕麦磷营养极为有利，因此从燕麦根际得到的2株有机磷细菌（YM-2，YM-4）值得进一步研究并开发利用。[著者文摘]
文章出处:
《干旱地区农业研究》-2007年25卷5期 -1-6页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1000-7601（2007）05-0001-06
Isolation of organic-phosphorus-dissolving bacteria in rhizosphere of oat （Avena nuda L. ） and their growth in cultural media
ZHENG Hong-li, ZHOU Xiao-rong, FAN Ming-shou（College of Agronomy, Inner Mongolia Agricultural University, Huhkot, Inner Mongolia 010019, China ）
Abstract:
Organic phosporus is an important P source in soil, but it is not able to be used by plant directly. Therefore, decomposing of organic phosphorus in soil is essential to its utilization by plants. Enhancing the decomposing of organic phosphorus in soil is of great significance in agriculture, and it has become a hot research topic in plant nutrition. Oat is an important crop in Inner Mongolia, phosphorus bioavailability is often a limiting factor for oat production. The main purpose of this research is to investigate if there is any bacteria in the rhizosphere of oat, which can dissolve the organic phosphorus. The results showed that five strains of organic- phosphorus-dissolving bacteria were isolated from rhizospheres of oat in Siziwang Inner mongolia. Two of them with higher capacity of dissolving organic phosphorus were selected for further study on their morphology and growth. Both YM-4 and YM-2 are bacilli without capsule and positive in gram reaction, after 4-5 days of culture, they all produced spores. Two strains, especially strain YM - 2 proved tolerant to salt in the experiment and grow well in media with 1% NaCl. The data on effects of different pH on the bacteria growth showed that neutral or alkaline culture media is prefered by the bacteria, acid media is not good for their growth. Along with the growth of both strains of bacteria, the media pH grdually decreases. This implies that growth and propagation of these two strains of bacteria in the soil can not only dissolve organic phosphorus, but probably also enhance the solubility of inorganic phosphates in alkine soil and therefore benefit P absorption by oat, which are usually planted in alkine soil. This suggests the strains of bacteria isolated from rhizosphere of oat may have great potential for use in practice. In this research, sorts of carbon resources were also compared in cultural media, the result showed that glucose is better than starch, sucrose, and lactose for the bacteria growth. This is important for slecting C resource when conducting propagation of the bacteria, and it also provides important information for utlization of these strains.[著者文摘]
Key words:
organic-phosphorus-dissolving bacteria; isolation; morphology; environmental factors
基金资助:
内蒙古自治区自然科学基金（200208020302,200607010302）
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