学术
“a”决定簇145位点变异的HBsAg单克隆抗体制备及HBV变异株快速检测方法
王军[1,2] 王继[1] 王丽[1]
[1]东北师范大学遗传与细胞研究所,长春130024 [2]长春市人民医院,长春130051
摘 要:
HBsAg抗原145位突变，可能导致临床上广泛使用的HBsAg诊断试剂出现漏检．通过杂交瘤技术制备出了一种抗该突变体的单克隆抗体，属IgG1亚类（κ型，效价为1：9×10^4）与其他类型肝炎病毒抗原无交叉反应．采用该单抗与多克隆抗体所建立的AC-ELISA法，对临床上乙型肝炎病毒（HBV）的突变样品进行检测的特异性和敏感性较高．[著者文摘]
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文章出处:
《生物化学与生物物理进展》-2008年35卷2期 -231-234页
栏目信息:
分 类 号:
Preparation of Monoclonal Antibody Against ＂a＂ Determinant With Mutation on Site 145 and Rapid Diagnosis Assay for HBV Mutant Strain
WANG Jun, WANG Ji, WANG Li（1.Institute of Genetics and Cytology, Northeast Normal University, Changehun 130024, China; 2.People＇s Hospital of Changehun, Changehun 130051, China）
Abstract:
Hepatitis B virus surface antigen with mutation on site 145 may cause omission in detection of hepatitis B virus with HBsAg diagnostic reagent. Monoclonal antibody against this mutation was obtained through hybridoma technique. The sub-class of the monoclonal antibody was determined to be IgG1 （κ type, the titer is 1 ： 9×10^4）, and the monoclonal antibody has no cross-reaction to other hepatitis virus. The method of AC-ELISA which was established with monoclonal antibody and polyclonal antibody showed high analysing susceptibility for HBV mutant samples.[著者文摘]
Key words:
hepatitis B virus surface antigen（HBsAg）, monoclonal antibody, HBV mutant strain, ELISA
基金资助:
吉林省科技发展计划项目（20060923-02）和长春市科技局资助项目（2005-120/05GG76）.
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