学术
高效液相色谱法检测人血浆中甲砜霉素及其药动学研究
黄成坷[1] 周伶俐[3] 邱相君[2] 陈冰冰[1] 娄旦[3] 胡国新[1]
[1]温州医学院药理学教研室,温州325027 [2]河南科技大学医学院药理学教研室,洛阳471003 [3]温州医学院附属第二医院,温州325027
摘 要:
目的：建立检测人血浆甲砜霉素的高效液相色谱法，并对其在人体内的药代动力学进行研究。方法：以ZORBAX SB—C18（4．6mm×250mm，5μm）为色谱柱；乙腈-水（30：70）为流动相，流速0．8mL·min^-1；以10，11-二氢-10-羟基-卡马西平（MHD）为内标，血浆样品经乙酸乙酯和正己烷提取后，在225am波长下进行检测。结果：甲砜霉素在0.1～10.0mg·L^-1浓度范围内线性关系良好（r=0．9999），定量下限为0．1mg·L^-1；0．25，1．0，5．0μg·mL^-1 3种浓度的日间、日内精密度RSD（n=5）均小于10％；相对回收率均在85％～115％范围内，绝对回收率大于75％。结论：本方法操作简便，结果准确；适用于血浆甲砜霉素的检测。[著者文摘]
文章出处:
《药物分析杂志》-2007年27卷2期 -177-179页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
0254-1793（2007）02-0177-03
HPLC determination of thiamphenicol in human plasma its pharmacokinetics in Chinese volunteers
HUANG Cheng - ke, ZHOU Ling - li, QIU Xiang - jun, CHEN Bing - bing, LOU Dan, HU Guo - xin. （ 1. Department of Pharmacology, Wenzhou Medical College, Wenzhou 325027, China; 2. Department of Pharmacology, Medical College of Henan University of Science and Technology, Luoyang 471003, China; 3. The Second Hospital of Wenzhou Medical College,Wenzhou 325027, China）
Abstract:
Objective：To establish an HPLC method for the determination of thiamphenicol in human plasma and study of its pharmacokinetics in Chinese volunteers. Method：The analytical column was packed with ZORBAX SB -C18 （4. 6 mm × 250 mm ,5 μm） ,the mobile phase was acetonitrile -water （30： 70） ,the flow rate was 0. 8 mL ·min^-1, and detection wavelength was set at 225 nm, respectively. Plasma samples were extracted with ethyl acetate and n - hexane. Results： The linear range of thiamphenicol in human plasma was 0. 1 - 10. 0 mg · L^-1 （ r = 0. 9999）. The lowest limit was 0. 1 mg · L^-1 ; Intra - day and inter - day RSD for the analysis of thiamphenicol in plasma samples at three concentration levels were both lower than 10%. Relative and absolute recoveries were 85% -115% and more than 75%, respectively. Conclusion： The HPLC method is simple, high selective and sensitive for the determination of thiamphenicol in plasma.[著者文摘]
Key words:
thiamphenicol; HPLC ; pharmacokinetics
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