学术
活性氧在UV-B诱导的玉米幼苗叶片乙烯产生中的作用
王弋博[1,2] 冯虎元[2] 曲颖[2] 程佳强[2] 王勋陵[2] 安黎哲[2,3]
[1]天水师范学院生物系,甘肃天水741000 [2]兰州大学生命科学学院,兰州730000 [3]中国科学院寒区旱区环境与工程研究所,兰州730000
摘 要:
研究了活性氧在UV-B（280～320nm）诱导的玉米（Zea mays）幼苗叶片乙烯合成中的作用。结果表明，UV-B促进了玉米幼苗活性氧和乙烯的产生；乙烯合成抑制剂氨氧乙烯基甘氨酸（AVG）和氨氧乙酸（AOA）能明显减弱UV—B对玉米幼苗乙烯产生的诱导作用，但对活性氧（ROS）的产生没有明显影响；ROS的清除剂不但能抑制UV-B诱导的ROS的产生，而且还可以抑制UV—B诱导的乙烯的产生，但这种抑制作用可以被外源O2^-的供体所逆转。这说明，乙烯的积累不能作为UV-B胁迫下ROS的诱导的因素，相反，ROS的积累则导致了乙烯的积累；因此，ROS可能参与了UV—B胁迫诱导的乙烯的产生。质膜NADPH氧化酶的抑制剂二苯碘销（DPI）和H2O2的特异性清除剂过氧化氢酶（CAT）对UV—B胁迫诱导的乙烯积累几乎没有影响，这说明H2O2可能与UV—B诱导的玉米幼苗叶片乙烯的产生无关，在UV—B诱导的玉米幼苗叶片乙烯的生物合成过程中O2^-起着很重要的作用，相关的O2^-不是由NADPH氧化酶催化产生的。[著者文摘]
文章出处:
《植物生态学报》-2007年31卷5期 -946-951页
栏目信息:
分 类 号:
EFFECTS OF REACTIVE OXYGEN SPECIES ON UV-B-INDUCED ETHYLENE PRODUCTION IN LEAVES OF MAIZE SEEDLINGS
WANG Yi-Bo, FENG Hu-Yuan, QU Ying, CHENG Jia-Qiang, WANG Xun-Ling, AN Li- Zhe（1.Department of Biology, Tianshui Normal College, Tianshui, Gansu 741000, China; 2.School of Life Sciences, Lanzhou University, Lanzhou 730000, China;3.Cold and Arid Region Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China）
Abstract:
Aims Ethylene accumulation and oxidative stress are two common responses of plants to environmental stresses; however, little is known about their relationships. Our objective was to investigate the role of reactive oxygen species （ROS） in ethylene synthesis induced by UV-B radiation （280 - 320 nm） in leaves of maize （ Zea mays ）. Methods Lamps were suspended above and perpendicular to the plastic trays and filtered with 0.13-mm thick cellulose diacetate （transmission down to 290 nm） for UV-B irradiance. The desired UV-B irradiation was obtained by changing the distance between the lamps and the plastic trays. The levels of UV-B irradiation were 4.8 kJ·m^-2·d^-1. Important findings UV-B radiation led to the generation of ROS and ethylene. The accumulation of ethylene induced by UV-B was not only inhibited by the scavengers of ROS, but also by aminoxyacetic acid （AOA） and 2-aminoethoxyvinlglycine （AVG）, which are specific inhibitors of ethylene synthesis. The inhibition effect of ROS scavengers on UV-B-induced ethylene production was reversed by O2^- donors. Results indicated that the increase in ethylene production may not be the cause of the increase in ROS production under UV-B stress. In contrast, the increase of ROS led to the accumulation of ethylene. Results suggested that ROS are involved in UV-B stress-induced ethylene accumulation. The accumulation of UV-B-induced ethylene was not affected by DPI, an inhibitor of NADPH oxidase and CAT, a specific scavenger of H2O2. Results suggested that the effect of H2O2 on UV-B-induced ethylene production in leaves of maize seedlings can be excluded, O2^- plays an important role in UV-B-induced ethylene synthesis in leaves of maize seedlings and O2^- serves as a potential me-diator of ethylene production that the plant can sense the UV-B stress. The related ROS are not from plasma membrane NADPH oxidase. The source of ROS contributing to ethylene accumulation under UV-B stress is unknown[著者文摘]
Key words:
reactive oxygen species （ROS）, ultraviolet-B radiation （UV-B）, ethylene production, maize
基金资助:
国家自然科学基金资助项目（30170083和30170186）
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