学术
摘 要:
目的探讨微波辐照诱导心肌细胞凋亡的分子病理机制。方法本实验以峰值功率密度950mW／cm^2（9500W／m^2）的脉冲微波辐照大鼠心肌细胞0、30、60、120s后，观察心肌细胞形态变化，检测心肌细胞凋亡率和多种凋亡相关基因的表达，以及心肌细胞内外Ca^2＋、钙调蛋白、钙通道蛋白的变化。结果辐照后心肌细胞呈现出典型的细胞凋亡形态。辐照60s后6、24h，心肌细胞凋亡率分别为（36．76±5．31）％和（26．44±3．94）％，与对照组（2．36±0．87）％相比较差异有显著性（P〈0．01），心肌细胞凋亡率与辐照剂量呈正相关。Bax、p53、c—myc表达显著增强（P〈0．01），Bcl-2表达一过性增强，随后明显降低（P〈0．01）。细胞内Ca^2＋明显降低，同时细胞外Ca^2＋显著升高。与对照组相比，钙调蛋白、钙通道蛋白的表达呈有规律下降性变化（P〈0．01），并与辐照时间呈正相关。结论心肌细胞是微波辐照损伤的敏感细胞，微波辐照可诱导心肌细胞凋亡，并存在剂量一效应关系。钙离子、钙调蛋白、钙通道蛋白以及多种基因参与微波辐照引起的心肌细胞凋亡，并发挥重要调控作用。[著者文摘]
文章出处:
《中国职业医学》-2007年34卷6期,455 -450-452,455页
栏目信息:
文献标识码:
A
文章编号:
1000-6486（2007）06-0450-04
Mechanism studies on Ca^2＋ and apoptosis of rat myocardial cells induced by microwave irradiation
DENG Hua, YANG Hong, LU Yu-kui, et al （College of Life Science, Foshan University , Foshan 528231, China）
Abstract:
Objective To study the molecular pathologic mechanism of myocardial cen apoptosis induced by microwave irradiation. Methods Rat myocardial cens were irradiated by pulse microwave at power density of 950 mW/cm^2 （9 500 W/m^2 ） , with the irradiation times being 0, 30, 60, and 120 s respectively. Then morphologic changes of cells were observed, and that apoptosis rate and the expression changes of various relevant apoptosis genes of myocardial cens were investigated. The changes of [ Ca^2 ＋ ], caleiphorin and calmodulin of myocardial cells were examined. Results Myocardial cells appeared representative apoptosis image after irradiation. Apoptosis rates of myocardial cens were 36.76 ± 5.31 and 26. 44 ± 3.94 respectively at 6 h and 24 h after irradiation. There was marked statistical discrepancy compared with the control group （ P 〈 0. 01 ）. The results indicated the dosage-effect relationship between apoptosis and irradiation dosage. The expression of Bax, p53, and c-myc enhanced obviously （P 〈 0.01 ） , and Bcl-2 enhanced transitorily, and then reduced obviously （P 〈 0. 01 ）. The concentration of cytoplasmic Ca^2＋ decreased significantly and extracellular [ Ca^2＋ ] increased significantly and simultaneously （P 〈 0.01 ）. Compared with the control group, the expression of calciphorin and calmodulin of myocardial cells changed obviously （ P 〈 0.01 ）. Conclusion Myocardial cells were susceptive to microwave irradiation. Irradiation could induce apoptosis of myocardial cells, showing dosage-effect relationship. Ca^2＋ ,calciphorin, calmedulin and many relevant apoptosis genes could be associated with apoptosis and could play important roles.[著者文摘]
Key words:
Microwave ; Myocardial cell ; Apoptosis ; Ca^2＋
基金资助:
广东省自然科学基金项目（04300712）
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