学术
摘 要:
目的探讨煤矿工人尘肺患者灌洗液中白细胞介素-12p70（IL-12p70）、IL-12p40、IL-18和IL-10水平及意义。方法用酶联免疫吸附双抗体夹心法检测59例尘肺患者（其中煤工尘肺40例、矽肺19例）灌洗波中IL-12p70、IL-12p40、IL-18和IL-10水平。结果煤工尘肺患者灌洗液IL-18水平随期别增加而明显下降；矽肺患者灌洗液IL-18水平随期别增加而上升，差异均具有显著性（P〈0．01和P〈0．05）。与Ⅱ、Ⅲ期煤工尘肺相比，Ⅱ、Ⅲ期矽肺患者灌洗液IL-18水平明显升高，差异有显著性（P〈0．01），IL-12．p70、IL-12p40和IL-10水平在各组中均无明显改变（P〉0．05）。与单纯煤工尘肺比较，煤工尘肺并发肺气肿患者灌洗液IL-10水平下降，差异有显著性（P〈0．05）；矽肺及其并发肺气肿患者灌洗液各项检测指标无显著改变。IL-12p70、IL-12p40和IL-10三者间呈正相关，与IL-18不相关。结论尘肺患者存在异常免疫应答。检测尘肺患者全肺灌洗液IL-18和IL-10水平可能对鉴别尘肺期别及判断疾病严重程度有一定价值。[著者文摘]
文章出处:
《中国职业医学》-2007年34卷6期 -472-474页
栏目信息:
分 类 号:
文献标识码:
B
文章编号:
1000-6486（2007）06-0472-03
Significance of detecting whole lung lavage fluid IL-12p70, IL-12p40, IL-18 and IL-10 in pneumocouiosis patients
YUAN Bao-jun, WANG Dong-mei, ZOU Ji-min, et al （ Department of Clinical Laboratory, Attached Kailuan Hospital of North China Coal Medical College, Tangshan 063000, China）
Abstract:
Objective To explore the significance of detecting whole lung lavage fluid IL-12p70, IL-12p40, IL-18 and IL-10 in pneumoeoniosis patients. Methods Lung lavage fluid levels of IL-12p70, IL-12p40, IL-18 and IL-10 were determined in 59 pneumoeoniosis patients [40 coal workers＇pneumoeoniosis （CWP） and 19 silicosis] by a sandwich ELISA. Results Lung lavage fluid levels of IL-18 in CWP were decreased with the stage going up, but in silicosis IL-18 levels were increased, and there were significantly statistical differenees（P 〈0. 01, P 〈 0. 05, respectively）. Compared with stage Ⅱ and Ⅲ CWP, lung lavage fluid levels of IL-18 in stage Ⅱ and Ⅲ silicosis were significantly higher（ P 〈 0.01 ） ; however no significant difference was observed in lung lavage fluid IL-12p70, IL-12p40 and IL-10 levels among the different groups（ P 〉 0. 05 ）. Lung lavage fluid levels of IL-10 in CWP combined with emphysema patients were Significantly lower than those in simple CWP patients （P 〈 0. 01 ）. IL-12p70, IL-12p40, IL-18 and IL-10 levels had no significant difference between silicosis and silicosis combined with emphysema patients. There were positive correlation among IL-12p70, IL-12p40 and IL-10, while there was no correlation among IL-12p70, IL-12p40, IL-10 and IL-18. Conclusion There exists abnomal immunoresponsiveness in pneumoeoniosis patients. The detection of the lung lavage fluid IL-18 and IL-10 may have certain value to distinguish the stages and judge the severity of pneumoeoniosis.[著者文摘]
Key words:
Pneumoconiosis; Lavage fluid; IL-12p70; IL-12p40; IL-18; IL-10
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