学术
氮离子束诱变提高黄嘌呤氧化酶产生菌产酶能力
夏小花[1] 李忠琴[1] 秦广雍[2] 王武[1]
[1]江南大学生物工程学院,江苏无锡214122 [2]郑州大学河南省离子束生物工程重点实验室,河南郑州450052
摘 要:
通过氮离子束诱变节杆菌（Arthrobacter sp．g-1984）筛选到黄嘌呤氧化酶高产菌株X7，产酶活力为24．49U／g，是出发株产酶活力（8．81U／g）的2．78倍，经发酵条件优化后，产酶活力达到36．67U／g，是出发菌株产酶活力的4．16倍，产酶高峰缩短了21h。对细菌黄嘌呤氧化酶酶学性质进行了初步研究，其最适反应条件为：37℃、pH6．5、Fe抖对酶活力有较强的抑制作用，EDTA对酶活力有明显的激活作用。[著者文摘]
文章出处:
《食品与生物技术学报》-2007年26卷6期 -81-85页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1673-1689（2007）06-0081-05
Mutagenesis of Arthrobacter sp. g-1984 for Raising the Production of Xanthine Oxidase
XIA Xiao-hua , LI Zhong-qin , QIN Guang-yong , WANG Wu （1. School of Biotechnology,Jiangnan University, Wuxi 214122, China; 2. Henan Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052,China）
Abstract:
Xanthine oxidase （XOD） producer Arthrobacter sp.g-1984 was mutagenized by N^＋ beam. A mutant strain X7 was screened, and produced XOD activity of 24.49 U/g , 2.78 times as the that of the parent strain （8.81 U/g）. After optimizing the fermentation conditions, the XOD yield reached at 36.67 U/g, 4.16 times as that of the parent strain, and the fermentation time of XOD was shortened by 21 h. Furthermore, its enzymatic properties were studied, and the results showed that the optimal temperature and pH for XOD reaction were 37℃ and pH 6.5 respetively. It was found that XOD activity was inhibited by Fe^2＋ but stimulated by EDTA.[著者文摘]
Key words:
xanthine oxidase;Arthrobacter; N^＋ ion beam mutagenesis; enzymatic properties
基金资助:
工业微生物技术教育部重点实验室基金项目（KL1B-KF200502）.
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