学术
摘 要:
研究了前体物质和碳氮源补加策略对钝齿棒杆菌Corynebacterium crenatumSYAN7发酵生产L-精氨酸的影响。结果表明，发酵24h后添加前体物质谷氨酸，以初始硫酸铵质量浓度20g／L，分别于24、48h补加20g／L硫酸铵的补氮方式，和以初始葡萄糖质量浓度100g／L，分别于24、48、72h均匀添加葡萄糖，使总糖质量浓度达175g／L的补碳方式，摇瓶发酵96h后，L-精氨酸产量最高达37．8g／L。[著者文摘]
文章出处:
《食品与生物技术学报》-2007年26卷6期 -86-89页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1673-1689（2007）06-0086-04
The Effect of Precursor and C ＆ N Source Feeding Tactics on L-Arginine Production by Corynebacterium crenatum Fermentation
XUE Feng, TA0 Wen-yi （Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China）
Abstract:
The effect of precursor and C ＆ N source feeding strategy on L-Arginine production by Corynebacterium crenatum SYAN7 was studied. The results showed that the yield of L-Arginine was increased to 37. 8 g/L by the combination of the fee.cling glutamic acid after 24 h, the feeding （NH4）2SO4in 24 h （20 g/L） and 48 h （20 g/L） with the initial （NH4）2SO4 concentration 20 g/ L, the feeding glucose in 24 h, 48 h and 72 h till the total glucose 175 g/L with the initial glucose concentration 100 g/L.[著者文摘]
Key words:
L-Arginine; Corynebacterium crenatum ; precursor; carbon source; nitrogen source
基金资助:
国家“十五”科技攻关重点项目（BE20001041）.
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