摘 要:
对利用基因重组技术获得的木薯醇腈酶(HNL)的酶学特异性进行研究,确定了其部分酶学性质。重组木薯HNL的最适温度为50℃,最适pH值为5.5。重组木薯HNL在60℃条件下,保温30min后仍具有90%的酶活力,说明温度稳定性较好;在pH4.0~6.0的条件下,获得的相对酶活仍大于80%。[著者文摘]
文章出处:
《食品与生物技术学报》-2007年26卷6期 -95-98页
Journal of Food Science and Biotechnology
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1673-1689(2007)06-0095-04
Studies on the Enzyme Property of Hydroxynitrile Lyas from Cassava
WANG Dan, LI Ya, ZHANG Tao, HE Lang (School of Basic Medical Sciences, Chengdu Medical College, Chengdu 610081,China)
Abstract:
The enzyme property of hydroxynitrile lyas which was obtained by gene engineering technology were studied in this manuscript. The part of enzyme properties were determined. The optimal condition of the enzyme was pH 5.5 and 50℃. Under 60℃, afer 30min the relative activity of recombinant hydroxynitrile lyase from cassava remained 90%. This iadicated the enzyme and pH 6. 0.[著者文摘]
Key words:
cassava; hydroxynitrile lyase; enzyme property
收稿日期: 2006-11-09
基金资助:
四川省青年基金项目(20068112).

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