学术
京2杨组培条件的优化及再生体系建立
张蕾[1] 苏晓华[2] 张冰玉[2] 黄秦军[2] 张香华[2] 李义良[2]
[1]沈阳农业大学林学院,辽宁沈阳110161 [2]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091
摘 要:
以京2杨为研究对象,确定了影响其组培苗生长的关键因子及组培苗生长的最佳条件,并为其建立了稳定高效的再生体系。试验结果表明：京2杨组织培养的最适基本培养基为MS培养基,继代培养基和不定芽最佳生根培养基均为1/2MS＋NAA 0.08 mg.L^-1＋IBA 0.08 mg.L^-1;培养基中加入活性碳不利于京2杨生长;光周期对京2杨的生长影响较小,但光照强度和继代培养基pH值对其生长影响显著,最适光照强度为2 500 lx,最适pH值为6.5;无菌苗叶片最佳不定芽诱导培养基为MS＋BA 1.0 mg.L^-1＋NAA 0.1 mg.L^-1,叶柄最佳不定芽诱导培养基为MS＋BA 1.0 mg.L^-1＋NAA 0.2 mg.L^-1。[著者文摘]
文章出处:
《林业科学研究》-2007年20卷6期 -787-793页
分 类 号:
文献标识码:
A
文章编号:
1001-1498（2007）06-0787-07
Optimization of Tissue Culture Conditions and Establishment of Regeneration System for Populus× euramericana cl. ‘J2＇
ZHANG Lei , SU Xiao-hua ,ZHANG Bing-yu, HUANG Qin-jun ,ZHANG Xiang-hua ,LI Yi-liang （ 1. Forestry College,Shenyang Agriculture University,Shenyang 110161 ,Liaoning,China; 2. Research Institute of Forestry, CAF; Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China）
Abstract:
The key factors affecting the growth of Populus × euramericana el. ‘J2＇ in vhro and the optimal tissue culture conditions for it were ascertained. The high frequency regeneration system for P.×euramericana cl. ‘J2＇ was also established in this study. The optimum basic culture medium was MS;the optimal subculture medium and root inducement culture medium for shoot were 1/2MS ＋ NAA 0.08 mg· L^-1 ＋ IBA 0.08·mg L^-1 ; active carbon disadvantaged the growth of P.× euramericana cl. ‘ J2＇ ; photoperiod had little effect on P. × euramericana cl. ‘ J2＇ , while light intensity and the pH value of subculture medium had great effect on it, the optimal light intensity was 2 500 Ix and the optimal pH value was 6.5 ; the optimal shoot inducement culture medium for leaf-explant was MS ＋ BA1.0 mg· L ^-1 ＋ NAA0.1 mg · L^-1 and for leafstalk-explant was MS ＋ BA 1.0 mg · L^-1 ＋ NAA 0.2 mg ·L^-1.[著者文摘]
Key words:
P. × euramericana cl.‘ J2＇ ; tissue culture ; regeneration system
基金资助:
国家科技支撑计划课题“高产优质杨树、桉树等速生材树种新品种选育”（编号2006BAD01A15）
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