学术
甜樱桃离体叶片再生的研究
付宏岐[1,2] 吴云锋[2] 张王斌[1,2]
[1]塔里木大学植物科技学院,新疆阿拉尔843300 [2]西北农林科技大学植保学院,陕西杨凌712100
摘 要:
利用甜樱桃的无菌苗叶片做外植体，进行离体叶片再生不定芽研究。结果表明：培养基中添加的激素浓度、种类和配比均影响不定芽再生，6-BA和NAA组合诱导甜樱桃离体叶片再生的活性较强；在MS＋6-BA3mg／L＋NAA0．2mg／L＋GA，0．5mg／L＋AgNO，5．0-10．0mg／L培养基上，通过3次继代培养的不定梢的叶片，再生不定芽的频率达6．4％，确定良好的生根培养基为1／2MS＋IBA0．7mg／L＋NAA0．2mg／L＋蔗糖20g／L＋琼脂7g／L，生根率可达75．1％。[著者文摘]
文章出处:
《塔里木大学学报》-2007年19卷1期 -13-16页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1009-0568（2007）01-0013-04
相关文章:
Research on Regeneration of Shoots from in Vitro Leaves of Sweet Cherry
Fu Hongqi Wu Yunfeng Zhang Wangbin （ 1 College of Plant Science and Technology, Tarim University, Alar, Xinjing 843300;2 College of Plant Protection, Northwest A＆F University, Yangling, Shaanxi 712100 ）
Abstract:
Studies with leaf discs from micro propagated axillaries shoots in sweat cherry show that different types,concentrations and combinations of hormones in the medium could influence regeneration of shoots. 6 - BA and NAA is more efficient than the others, 3 subcultures of leaf discs from adventitious shoots on the medium amended with MS ＋ 6 - BA3mg/L ＋ NAA0.2mg/L ＋ GA30.5mg/L ＋ AgNO35.0 - 10.0mg/L gave 6.4% of regeneration frequency, Meanwhile, the inducing rate on culture medium of 1/2MS ＋ IBA 0. 7mg/L ＋ NAA0. 2mg/L ＋ sucrose 20 g/L ＋ agar 7g/L being responsible for root growth up to 75.1% were accounted for the optimum one.[著者文摘]
Key words:
sweet eherry; tissue euhure; in virto leaf; adventitious bud
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