学术
利用菌落PCR—DGGE快速鉴定盐渍土壤菌种多样性
张海燕 贺江舟 龚明福 刘占文 张利莉
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300
摘 要:
利用菌落PCR方法，结合DGGE的检测方法，对所保存的87份盐生菌的培养物进行了多样性检测，初步研究显示至少有31株不同的菌种，表明新疆盐生环境存在较为丰富的菌种资源，同时表明菌落PCR结合DGGE技术为细菌鉴定和菌种保藏过程中重复菌株的去除提供了一种快速可靠的手段。[著者文摘]
文章出处:
《塔里木大学学报》-2007年19卷4期 -30-32页
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1009-0568（2007）04-0030-03
相关文章:
Based on Bacterial Colony PCR- DGGE for Rapid Determination of Microbial Diversity in Xinjiang Salt- soil
Zhang Haiyan, He Jiangzhou, Gong Mingfu ,Liu Zhanwen ,Zhang Lili （ Key Laboratory of Protection ＆ Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Groups, Tarim University, Alar, Xinjiang, 843300 ）
Abstract:
In this paper, the combination of bacterial colony PCR and denaturing gradient electrophoresis were used in the microbial diversity analysis of 87 halo inhabited bacterial culture collections. The results showed the recovered 87 culture collections contain at least 31 different strains, which confirmed that the combination of colony PCR and DGGE is a reliable and time -conserved approach to eliminate the repetitive strains in bacterial identification and strain conservation. The results showed there was great microbial diversity in salt -soil in Xinjiang.[著者文摘]
Key words:
bacterial colony PCR; DGGE; diversity; strain conservation
基金资助:
塔里木大学校长基金（编号：TDZKZD06001）;新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室开发课题（编号：RB0606）;塔里木大学校长基金重点资助项目（编号：2004-2）
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