学术
PCR-RFLP分析用的梨基因组DNA提取
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辜青青[1,2] 罗正荣[1]
[1]华中农业大学园艺植物生物学教育部重点实验室,湖北武汉430070 [2]江西农业大学农学院,江西南昌330045
国际标准刊号：ISSN 1000-2286
国内统一刊号：CN 36-1028
摘 要:
采用改良CTAB法,提取了23个梨品种基因组DNA。结果表明：所提取DNA产率在200μg/g鲜叶以上,大部分梨品种OD260/OD280在1.8左右,用于PCR-RFLP分析时,PCR扩增产物谱带清晰,且PCR扩增产物能被相应的限制性内切酶酶切。[著者文摘]
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文章出处:
《江西农业大学学报》-2007年29卷5期 -736-739页
Acta Agriculturae Universitis Jiangxiensis
栏目信息:
分 类 号:
文献标识码:
A
文章编号:
1000-2286（2007）05-0736-04
[参考文献]
Genomic DNA Extraction for PCR-RFLP Analysis Pear （Pyrus pyrifolia Nakai）
GU Qing-qing, LUO Zheng-rong. （1.Key Laboratory of （Ministry of Education） for Horticultural Plant Biology,Huazhong Agricuhural University, Wuhan 430070,China; 2.College of Agronomy, JAU,Nanchang 330045 ,China）
Abstract:
The genomic DNA of 23 pear cultivars was extracted from young leaves by an improved CTAB method. The average yield of DNA was over 200 μg/g by fresh leaves. The value of OD260/ OD280 for most pear cultivars ranged from 1.7 to 1.9. These results indicated that the purity of the extracted DNA was suitable for PCR-RFLP analysis. The PCR amplification profiles were distinct. The PCR fragments could be digested with corresponding restriction endonucleases.[著者文摘]
Key words:
Pyrus pyrifolia Nakai; DNA isolation; CTAB method;PCR-RFLP analysis
收稿日期: 2007-07-03
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